Related Experiment Video
Updated: Feb 15, 2026

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract
Published on: June 17, 2014
[HUVECs respond to low shear stress stimulation by activating Wnt/β-catenin signaling pathway]
Xin Sheng1, Yuehua Liu2, Junhua Wang2
1Department of Biochemistry, Zunyi Medical University, Zunyi 563000, China. *Corresponding author,
Abstract:
Objective To investigate the influence of different fluid shear stress (FSS) on Wnt/β signaling pathway in human umbilical vein endothelial cells (HUVECs) and its relationship with atherosclerosis. Methods HUVECs were subjected to different FSS (0, 1, 15 dynes/cm2) for various durations (6, 12, 18, 24 hours) using a shear stress device. Subsequently, real-time quantitative PCR was used to observe the mRNA expression levels of dishevelled 2 (Dvl2) and β-catenin in the Wnt signaling pathway, and immunofluorescence technology to observe their protein expression and localization. Results The low FSS promoted the expression and recruitment of Dvl2 as well as the translocation of β-catenin into the nucleus at the early stage of loading low FSS. Conversely, laminar FSS inhabited their expression and nuclear translocation of β-catenin. Conclusion Wnt signaling pathway participates in the stress response of vascular endothelial cells to low shear stress.
Related Concept Videos
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathways
TGF - β Signaling Pathway
Shearing Stress
The average shearing stress can be calculated by dividing the shear by the area of the cross-section.
Shearing Stresses in a Beam: Problem Solving
Catenins
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...

