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Updated: Feb 15, 2026

Investigating Teliospore Germination Using Microrespiration Analysis and Microdissection
Published on: May 13, 2018
Isolation and Analysis of Xenopus Germinal Vesicles
1School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, United Kingdom garry.morgan@nottingham.ac.uk.
Abstract:
The giant nucleus or germinal vesicle (GV) of Xenopus oocytes provides an unusual opportunity to analyze nuclear structure and function in exquisite detail by light microscopy. Detailed here are two rapid procedures for using manually isolated GVs in combination with fluorescent reporter proteins to investigate the lampbrush chromosomes and nuclear bodies of oocytes. One procedure provides spreads of nuclear components in an unfixed and life-like, although not living, form. The other describes the isolation of intact, functional GVs directly into mineral oil offering possibilities for direct observation of nuclear dynamics.
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