Related Experiment Video

Updated: Feb 15, 2026

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples
09:36

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Published on: July 2, 2016

17.7K

Long non-coding RNA deep sequencing reveals the role of macrophage in liver disorders

Zhang Lin1,2,3, Hao Changfu1, Zhao Fengling4

  • 1Department of Occupational and Environmental Health, School of Public Health, Zhengzhou University, Zhengzhou 450001, China.

Oncotarget
|February 1, 2018
PubMed

Insights

This study reveals long non-coding RNAs (lncRNAs) shared between macrophages and liver tissue in liver disorders. These differentially expressed lncRNAs highlight macrophages

Area of Science:

  • Immunology
  • Molecular Biology
  • Hepatology

Background:

  • Liver disorders like hepatitis, cirrhosis, and hepatocellular carcinoma involve significant inflammation.
  • Macrophages are critical regulators and initiators of inflammatory processes.
  • Long non-coding RNAs (lncRNAs) are implicated in various disease developments.

Purpose of the Study:

  • To investigate the role of macrophages in liver disorders using lncRNA sequencing.
  • To identify shared lncRNAs between macrophages and liver tissue in the context of liver disease.
  • To explore the regulatory mechanisms of these lncRNAs.

Main Methods:

  • Applied a lncRNA classification pipeline to identify 1247 lncRNAs.
  • Analyzed lncRNA expression patterns in gene expression profiles of silica-exposed macrophages and liver.
  • Identified and validated differentially expressed lncRNAs shared between macrophages and liver.
  • Performed functional enrichment analysis based on expression signatures (cis, trans, co-acting proteins).

Main Results:

  • Identified 1247 lncRNAs using a classification pipeline.
  • Discovered differentially expressed lncRNAs common to both macrophages and liver tissue.
  • Functional enrichment analysis proposed cis, trans, and co-acting protein regulatory aspects.
  • Established a novel correlation between macrophages and liver disorders via co-expressed lncRNAs.

Conclusions:

  • Macrophage roles in liver disorders are complex and potentially dual-edged.
  • Differentially expressed lncRNAs and their associated genes/proteins may serve as diagnostic biomarkers.
  • These lncRNAs represent potential therapeutic targets for liver disorders.
Keywords:
biomarkercancerliver disorderlncRNA profilingmacrophage

More Related Videos

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

26.4K
Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood09:40

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood

Published on: November 28, 2018

7.8K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.0K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
13.4K
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.7K
RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.9K
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.8K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
14.9K