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Adipose-derived stem cells decrease cardiomyocyte damage induced by porphyromonas gingivalis endotoxin through
Tung-Sheng Chen1,2, Chia-Hua Kuo3, Sarnai Battsengel2,4
1School of Life Science, National Taiwan Normal University, Taipei, Taiwan.
Environmental Toxicology
|February 1, 2018
Summary
Periodontal disease may lead to heart failure. Adipose-derived stem cells (ADSC) protect heart cells from damage caused by Porphyromonas gingivalis endotoxin (Pg-LPS), potentially via IGF1 signaling.
Area of Science:
- Cardiovascular Science
- Periodontology
- Regenerative Medicine
Background:
- Periodontal disease is linked to heart failure complications.
- Stem cell therapy is a promising treatment for cardiomyopathy.
- Porphyromonas gingivalis endotoxin (Pg-LPS) can induce cardiomyocyte damage.
Purpose of the Study:
- To investigate the protective effects of stem cells on cardiomyocyte damage induced by Pg-LPS.
- To explore the mechanisms underlying stem cell-mediated cardioprotection.
Main Methods:
- Cardiomyocytes were exposed to Pg-LPS.
- Co-culture experiments with adipose-derived stem cells (ADSC) were performed.
- Apoptosis, hypertrophy, fibrosis, MAPK signaling, and IGF1 levels were assessed.
Main Results:
- Pg-LPS reduced cardiomyocyte viability by activating apoptosis, hypertrophy, fibrosis, and MAPK signaling.
- ADSC co-culture increased cardiomyocyte viability by suppressing these pathological markers.
- Insulin-like growth factor 1 (IGF1) secreted by ADSC was identified as a key survival marker.
Conclusions:
- ADSC demonstrate significant cardioprotective effects against Pg-LPS-induced damage.
- ADSC suppress key pathological pathways including apoptosis, hypertrophy, fibrosis, and MAPK signaling.
- IGF1 secreted by ADSC plays a crucial role in mitigating cardiomyocyte damage.
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