Related Experiment Video
Updated: Feb 15, 2026

Author Spotlight: Advancing Gene Editing in Bamboo Leaves for Sustainable Plastic Alternatives
Published on: August 18, 2023
Two G-box-like elements essential to high gene expression of SlAKR4B in tomato leaves
Marina Suekawa1, Yukichi Fujikawa1, Muneharu Esaka1
1a Graduate School of Biosphere Sciences , Hiroshima University , Higashi-Hiroshima , Japan.
Abstract:
Aldo-keto reductases (AKRs) play important roles in aldehyde detoxification as well as primary and secondary metabolism in plants. We previously reported inducible expression of a Solanum lycopersicum AKR4B (SlAKR4B) in tomato leaves treated with salicylic acid and jasmonic acid, and high promoter activity of SlAKR4B in tomato leaf protoplasts. In this study, we investigated the expression response of SlAKR4B in the tomato leaves with infiltration treatment and the cis-element(s) involved in high promoter activity. Gene expression analysis in tomato leaf protoplasts and buffer-infiltrated tomato leaves suggested that cell damage caused the increased expression of SlAKR4B. Promoter activity of SlAKR4B was significantly reduced by mutation of two G-box like elements. It is suggested that the two G-box like elements are responsible for the high promoter activity.
Related Concept Videos
What is Gene Expression?
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
What is Gene Expression?
Cell Specific Gene Expression
Chromatin Position Affects Gene Expression
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Key Elements for Plant Nutrition
mRNA Stability and Gene Expression
Cis-acting Elements involved in mRNA stability

