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High-Throughput Metabolic Profiling for Model Refinements of Microalgae
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A gas trapping method for high-throughput metabolic experiments.

James R Krycer1, Ciana Diskin1, Marin E Nelson1

  • 1School of Life and Environmental Sciences, Charles Perkins Centre, The University of Sydney.

Biotechniques
|February 1, 2018
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel gas trapping protocol for multiwell plates to measure gaseous products like carbon dioxide from cellular metabolism. This cost-effective method supports high-throughput metabolic research with both adherent and suspension cells.

Keywords:
adipocytecarbon dioxidecell metabolismgas traphydrogen sulfide

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Metabolic Research

Background:

  • High-throughput cellular metabolism research commonly uses multiwell plates.
  • Collecting gaseous products (e.g., carbon dioxide) from these plates is challenging due to the need for sealed environments.
  • Existing methods are often not compatible with the demands of high-throughput screening.

Purpose of the Study:

  • To develop a simple, cost-effective gas trapping protocol for multiwell plates.
  • To enable the measurement of gaseous metabolites in high-throughput cell culture experiments.
  • To facilitate the study of cellular respiration and other metabolic processes involving gas production.

Main Methods:

  • A novel gas trapping system was designed using perforated plastic lids for sealed cell-culture multiwell plates.
  • The protocol was validated by measuring carbon dioxide production from glucose and fatty acid metabolism.
  • Hydrogen sulfide production from cysteine-treated cells was also quantified.

Main Results:

  • The gas trap effectively collected gaseous products in both liquid and solid media.
  • The method successfully quantified carbon dioxide and hydrogen sulfide production.
  • The protocol is applicable to both adherent and suspension cell cultures.

Conclusions:

  • The developed gas trap protocol is a convenient and cost-effective solution for measuring gaseous products in high-throughput metabolic research.
  • This method is adaptable to various multiwell plate formats, supporting the increasing trend towards large-scale cellular analysis.
  • The protocol enhances the capability of studying cellular metabolism in a high-throughput manner.