Related Experiment Video
Updated: Feb 15, 2026
09:39
Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry
Published on: March 17, 2015
24.0K
New Technologies for Analysis of Extracellular Vesicles
Huilin Shao1,2,3, Hyungsoon Im4,5, Cesar M Castro4,6
1Departments of Biomedical Engineering and Surgery, National University of Singapore , Singapore 117583.
Chemical Reviews
|February 1, 2018
Summary
Extracellular vesicles (EVs), crucial for cell communication and disease biomarkers, face challenges in clinical use due to limited detection technologies. This review explores new analytical methods to advance EV research and applications.
Area of Science:
- Cell Biology
- Biochemistry
- Nanotechnology
Background:
- Extracellular vesicles (EVs) are nanoscale membrane vesicles actively released by cells.
- EVs mediate intercellular communication and serve as biomarkers for disease diagnosis and prognosis.
- Current limitations in EV detection technologies hinder clinical translation.
Purpose of the Study:
- To provide a comprehensive overview of emerging analytical technologies for EV detection.
- To critically evaluate these technologies and their potential clinical applications.
- To highlight recent advances impacting basic and translational EV research.
Main Methods:
- Review of current literature on EV analytical technologies.
- Focus on molecular and other advanced detection platforms.
- Analysis of technological challenges and opportunities.
Main Results:
- Emerging analytical platforms offer improved sensitivity and specificity for EV detection.
- These technologies are crucial for overcoming barriers to clinical translation.
- Advances are poised to significantly impact both fundamental research and clinical practice.
Conclusions:
- New analytical technologies are essential for unlocking the full potential of EVs in diagnostics and therapeutics.
- Continued development is needed to standardize EV analysis and facilitate clinical adoption.
- The field of EV research is rapidly advancing, promising significant future breakthroughs.
Related Concept Videos
01:42The Extracellular Matrix
89.6K
Overview
89.6K
01:29The Extracellular Matrix
12.5K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
12.5K
01:31Hybridoma Technology
17.8K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
17.8K
01:30Health Information Technology and Healthcare Information System
3.4K
Health Information Technology (HIT)
Health Information Technology, commonly called HIT, integrates advanced information systems and technology in healthcare settings. Its primary functions include:
Health Information Technology, commonly called HIT, integrates advanced information systems and technology in healthcare settings. Its primary functions include:
3.4K
01:05Insulin Secretory Vesicles
7.0K
Insulin secretory vesicles release insulin to stimulate blood glucose uptake and regulate carbohydrate metabolism. When the blood glucose levels increase, glucose enters the pancreatic β-islet cells through glucose transporters. Once inside, glucose is metabolized through glycolysis, the citric acid cycle, and the electron transport chain, producing ATP. This increase in ATP concentration closes ATP-sensitive potassium channels, leading to depolarization of the membrane and the opening of...
7.0K
01:33Overview of Secretory Vesicles
9.6K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
9.6K