Related Experiment Video
Updated: Feb 15, 2026

Visualizing Early Infection Sites of Rice Blast Disease Magnaporthe oryzae on Barley Hordeum vulgare Using a Basic Microscope and a Smartphone
Published on: March 17, 2023
Selection of Differential Isolates of Magnaporthe oryzae for Postulation of Blast Resistance Genes
W W Fang1, C C Liu1, H W Zhang1
1First, second, third, fifth, sixth, seventh, and eighth authors: State Key Laboratory of Agrobiotechnology and MOA Key Lab of Pest Monitoring and Green Management, China Agricultural University, Beijing 100193, China; and fourth author: Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China.
Abstract:
A set of differential isolates of Magnaporthe oryzae is needed for the postulation of blast resistance genes in numerous rice varieties and breeding materials. In this study, the pathotypes of 1,377 M. oryzae isolates from different regions of China were determined by inoculating detached rice leaves of 24 monogenic lines. Among them, 25 isolates were selected as differential isolates based on the following characteristics: they had distinct responses on the monogenic lines, contained the minimum number of avirulence genes, were stable in pathogenicity and conidiation during consecutive culture, were consistent colony growth rate, and, together, could differentiate combinations of the 24 major blast resistance genes. Seedlings of rice cultivars were inoculated with this differential set of isolates to postulate whether they contain 1 or more than 1 of the 24 blast resistance genes. The results were consistent with those from polymerase chain reaction analysis of target resistance genes. Establishment of a standard set of differential isolates will facilitate breeding for blast resistance and improved management of rice blast disease.
More Related Videos
08:58Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
07:14The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
Published on: August 4, 2018
Related Concept Videos
Antibiotic Selection
Basic Postulates of Kinetic Molecular Theory: Particle Size, Energy, and Collision
Gene Flow
Resistivity
Resistance
What is Natural Selection?