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Published on: March 20, 2019
Distinct gibberellin functions during and after grapevine bud dormancy release
Chuanlin Zheng1, Atiako Kwame Acheampong1, Zhaowan Shi1,2
1Institute of Plant Sciences, Department of Fruit Tree Sciences, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel.
Gibberellin (GA) levels are restricted during initial grapevine bud activation but increase to promote regrowth after meristem activation. This study clarifies GA
Area of Science:
- Plant Physiology
- Molecular Biology
- Horticulture
Background:
- Grapevine bud dormancy release mechanisms are not fully understood.
- Previous hypotheses suggest abscisic acid (ABA) repression and ethylene-ABA interplay.
- The role of gibberellins (GAs) in dormancy release requires further investigation.
Purpose of the Study:
- To examine the involvement of gibberellins (GAs) in grapevine bud dormancy release.
- To investigate the expression of GA metabolism genes during dormancy induction and release.
- To determine the effect of GA application and hydrogen cyanamide on bud break.
Main Methods:
- Gene expression analysis of VvGA3ox, VvGA20ox, VvGASA2, and VvGA2ox transcripts.
- Quantification of GA1 levels during dormancy.
- Application of GA and hydrogen cyanamide to grapevine buds.
Main Results:
- GA metabolism gene expression and GA1 levels decreased during dormancy induction and increased during release.
- GA application inhibited bud break, while hydrogen cyanamide partially rescued this effect.
- GA application showed inhibitory effects only when applied during bud-forcing initiation.
Conclusions:
- GA levels must be restricted during initial dormant bud meristem activation.
- Increased GA levels after meristem activation enhance primordia regrowth.
- A precise regulation of GA metabolism is crucial for grapevine bud dormancy release.
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