Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intrinsically Disordered Proteins02:18

Intrinsically Disordered Proteins

19.6K
Intrinsically disordered proteins are a group of proteins that do not fold into specific three-dimensional structures. Their structural flexibility allows them to complement ordered proteins to perform functions that are inaccessible to rigid structures. They are more common in eukaryotes than prokaryotes and may either be exclusively intrinsically disordered or hybrid proteins, consisting of a mix of ordered and disordered regions. The absence of a rigid structure in these proteins can be...
19.6K
Intrinsically Disordered Proteins02:18

Intrinsically Disordered Proteins

2.9K
2.9K
Extrinsic and Intrinsic Pathways of Hemostasis01:20

Extrinsic and Intrinsic Pathways of Hemostasis

13.3K
Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
13.3K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

8.7K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
8.7K
Cross-Sectional Research01:50

Cross-Sectional Research

12.7K
In cross-sectional research, a researcher compares multiple segments of the population at the same time. If they were interested in people's dietary habits, the researcher might directly compare different groups of people by age. Instead of following a group of people for 20 years to see how their dietary habits changed from decade to decade, the researcher would study a group of 20-year-old individuals and compare them to a group of 30-year-old individuals and a group of 40-year-old...
12.7K
Method of Sections01:30

Method of Sections

1.3K
Consider a truss structure, as shown in the figure.
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transient photocyclization in ruthenium(ii) polypyridine complexes of indolamines.

Physical chemistry chemical physics : PCCP·2017
Same author

In vivo photorelease of GABA in the mouse cortex.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2011
Same author

Electrical Communication between Electrodes and Enzymes Mediated by Redox Hydrogels.

Analytical chemistry·2011
Same author

Layer-by-layer self-assembly of glucose oxidase and Os(Bpy)2CIPyCH2NH-poly(allylamine) bioelectrode.

Analytical chemistry·2001

Related Experiment Video

Updated: Feb 15, 2026

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
12:51

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

10.4K

Intrinsic optical sectioning with upconverting nanoparticles.

C Sorbello1, R Etchenique

  • 1Departamento de Química Inorgánica, Analítica y Química Física, INQUIMAE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, CONICET, Ciudad Universitaria Pabellón 2, AR1428EHA Buenos Aires, Argentina. rober@qi.fcen.uba.ar.

Chemical Communications (Cambridge, England)
|February 2, 2018
PubMed
Summary

Lanthanide-based Upconverting Nanoparticles provide an affordable alternative to expensive multiphoton microscopy. This breakthrough enables deep-tissue optical sectioning in 3D samples for advanced bioimaging.

More Related Videos

Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

23.0K
Analyzing the Movement of the Nauplius 'Artemia salina' by Optical Tracking of Plasmonic Nanoparticles
05:52

Analyzing the Movement of the Nauplius 'Artemia salina' by Optical Tracking of Plasmonic Nanoparticles

Published on: July 15, 2014

11.0K

Related Experiment Videos

Last Updated: Feb 15, 2026

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
12:51

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

10.4K
Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

23.0K
Analyzing the Movement of the Nauplius 'Artemia salina' by Optical Tracking of Plasmonic Nanoparticles
05:52

Analyzing the Movement of the Nauplius 'Artemia salina' by Optical Tracking of Plasmonic Nanoparticles

Published on: July 15, 2014

11.0K

Area of Science:

  • Biophotonics
  • Nanotechnology
  • Microscopy

Background:

  • Multiphoton microscopy offers deep tissue penetration and optical sectioning capabilities.
  • High cost of femtosecond lasers limits accessibility of multiphoton microscopy.
  • Need for cost-effective advanced imaging solutions in biological research.

Purpose of the Study:

  • To introduce lanthanide-based Upconverting Nanoparticles (UCNPs) as a low-cost alternative for multiphoton imaging.
  • To demonstrate the feasibility of UCNPs for direct optical sectioning in complex 3D biological samples.
  • To overcome the cost barrier associated with traditional multiphoton microscopy.

Main Methods:

  • Utilized lanthanide-based Upconverting Nanoparticles (UCNPs) for optical imaging.
  • Investigated the deep penetration and low scattering properties of UCNPs.
  • Demonstrated direct optical sectioning capabilities in 3D sample environments.
  • Assessed the cost-effectiveness of the UCNP-based approach (under $1000).

Main Results:

  • UCNPs provide an affordable solution for advanced imaging, costing under $1000.
  • Achieved direct optical sectioning in complex 3D samples using UCNPs.
  • Maintained key advantages of multiphoton microscopy, including deep penetration and low scattering.
  • UCNPs enable precise control for both imaging and molecular actuation.

Conclusions:

  • Lanthanide-based UCNPs present a viable and cost-effective alternative to conventional multiphoton microscopy.
  • This technology democratizes advanced optical sectioning for complex biological imaging.
  • UCNPs offer a promising platform for future developments in bioimaging and molecular actuation.