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Related Experiment Video

Updated: Feb 15, 2026

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Analyzing Mouse B Cell Responses Specific to LCMV Infection.

Yaxing Hao1, Zhirong Li1, Yifei Wang1,2

  • 1Institute of Immunology, Third Military Medical University, Gaotanyan, Shapingba District, Chongqing, 400038, China.

Methods in Molecular Biology (Clifton, N.J.)
|February 2, 2018
PubMed
Summary

This study details a protocol for analyzing B cell immune responses to lymphocytic choriomeningitis virus (LCMV) infection in mice. It provides methods to track germinal center B cells, viral antibodies, and antibody-secreting plasma cells, crucial for vaccine development.

Keywords:
AntibodyELISAELISPOTFlow cytometryGerminal centerLCMVPlasma cellsTFH cellsTetramer

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Area of Science:

  • Immunology
  • Virology
  • Vaccinology

Background:

  • B cell responses are critical for humoral immunity against viral pathogens.
  • Understanding B cell dynamics during viral infections is vital for developing effective antiviral vaccines.
  • The lymphocytic choriomeningitis virus (LCMV) model in mice elicits typical T cell-dependent B cell responses.

Purpose of the Study:

  • To describe a comprehensive protocol for studying anti-viral B cell responses.
  • To detail methods for establishing and quantifying acute LCMV infection in mice.
  • To provide techniques for analyzing specific B cell populations and antibody production.

Main Methods:

  • Propagation and quantification of LCMV for controlled infection.
  • Flow cytometry to identify germinal center (GC) B cells and follicular helper T (TFH) cells.
  • Enzyme-linked immunosorbent assay (ELISA) for quantifying LCMV-specific IgG antibodies in serum.
  • Enzyme-linked immunospot (ELISPOT) assay to detect LCMV-IgG secreting plasma cells in bone marrow.

Main Results:

  • Successful establishment of acute LCMV infection in mice.
  • Quantification of LCMV-specific IgG antibody titers in serum.
  • Detection and enumeration of antibody-secreting plasma cells in bone marrow.
  • Identification of GC B cells and TFH cells in spleen and lymph nodes.

Conclusions:

  • The described protocol provides a robust framework for investigating anti-viral B cell immunity.
  • This model system facilitates detailed analysis of humoral immune responses to viral infections.
  • The methods are essential for advancing research in antiviral vaccine design and efficacy.