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Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors

Zhiyong Yang1,2, Christopher D C Allen3,4,5

  • 1Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA, 94143, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 2, 2018
PubMed

B cells, after activation, can undergo class-switch recombination and somatic hypermutation of their immunoglobulin genes, and can differentiate into memory cells and plasma cells. Expressing genes in altered versions in primary B cells and B cell lines is an important approach to understanding how B cell receptor signaling leads to B cell activation and differentiation. Recombinant retrovirus-based transduction is the most efficient method to deliver exogenous genes for expression in B cells. In this chapter, we describe streamlined protocols for using recombinant retroviral vectors to transduce both murine primary B cells and B cell lines.

Keywords:
B cell linesExogenous gene expressionPrimary B cellRetroviral vectorSpinfectionTransduction

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