Related Experiment Video
Updated: Feb 15, 2026
Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy
Published on: August 9, 2019
In Vivo Tracking of Particulate Antigen Localization and Recognition by B Lymphocytes at Lymph Nodes
1B Cell Dynamics Laboratory, Department of Immunology and Oncology, Centro Nacional de Biotecnología (CNB)-CSIC, Darwin 3, UAM-Campus Cantoblanco, Madrid, 28049, Spain. ycarrasco@cnb.csic.es.
The development of experimental systems that allow in vivo antigen tracking as well as the study of B cell dynamics in real time and in situ, have transformed our understanding of the "how, when and where" B lymphocytes find antigen at secondary lymphoid organs in the last 10 years. Here, I described one of these experimental models, which uses highly fluorescent particulate antigen and B cell receptor (BCR)-transgenic B cells labeled with long-term fluorescent probes, combined with confocal and multiphoton microscopy.
The development of experimental systems that allow in vivo antigen tracking as well as the study of B cell dynamics in real time and in situ, have transformed our understanding of the "how, when and where" B lymphocytes find antigen at secondary lymphoid organs in the last 10 years. Here, I described one of these experimental models, which uses highly fluorescent particulate antigen and B cell receptor (BCR)-transgenic B cells labeled with long-term fluorescent probes, combined with confocal and multiphoton microscopy.
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