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Updated: Feb 15, 2026

Quantification of Circular RNAs Using Digital Droplet PCR
Published on: September 16, 2022
Droplet Digital PCR for Quantitation of Extracellular RNA
Irene K Yan1, Rishabh Lohray1, Tushar Patel2
1Department of Transplantation, Mayo Clinic, Jacksonville, FL, USA.
Abstract:
Cell-to-cell communication involves the release of biological molecules into the extracellular space and their uptake by recipient cells. These molecules include RNA that can modulate cellular signaling and biological processes. To study extracellular RNA, highly sensitive and precise methods for their detection are needed. Digital polymerase chain reaction (dPCR) can be a useful method for detecting and analyzing extracellular RNA. The sensitivity of digital PCR can exceed that of quantitative PCR for low abundance targets such as extracellular RNA.
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