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Updated: Feb 15, 2026

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
Published on: September 14, 2013
A novel method for the transport and long-term storage of cultures and samples in an anaerobic atmosphere
B A Cragg1, S J Bale1, R J Parkes1
1Geomicrobiology Unit, Department of Geology, University of Bristol, Bristol BS8 1RJ.
Abstract:
Nylon-aluminium-polyethylene laminated, sealable bags provided a more economical and flexible method of transportation, incubation and storage than anaerobic jars. The bags were used with a special heat sealer and 'Anaerocult-A' sachets to remove oxygen. The environment inside the bags remained oxygen-free for at least 3 years.
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