Transcriptomic profiling of Clostridium difficile grown under microaerophillic conditions
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Updated: Feb 15, 2026

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
Published on: September 14, 2013
Nicole Giordano1, Jessica L Hastie1, Paul E Carlson1
1Center for Biologics Evaluation and Research, Office of Vaccines Research and Review, Division of Bacterial, Parasitic and Allergenic Products, US Food and Drug Administration, Silver Spring, MD 20993, USA.
Abstract:
Clostridium difficile (Cd) is an anaerobic, spore-forming bacterium capable of colonizing the gastrointestinal tract of humans. Colonization usually occurs following antibiotic-induced disruption of the host microbiota, which also leads to an increase in oxygen within the gastrointestinal tract. We sought to understand how Cd responds to this microaerophilic condition that is likely experienced within the host. Transcriptome profiling showed differential regulation of genes involved in sugar metabolism, pyruvate metabolism and stress responses. These data provide insight into potential mechanisms of Cd adaptation to the host environment and should lead to the elucidation unknown mechanisms of Cd oxygen resistance and pathogenesis.
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