Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assessment of Diffusion and Perfusion01:17

Assessment of Diffusion and Perfusion

1.7K
Understanding and evaluating diffusion and perfusion is critical in assessing a patient's respiratory and circulatory health. These processes play key roles in maintaining the body's internal environment, ensuring that tissues receive adequate oxygen while waste products are efficiently removed.
The Role of Diffusion in Respiration
Diffusion is the process by which molecules move from an area of higher concentration to an area of lower concentration. In the respiratory system, this...
1.7K
Gene Flow02:39

Gene Flow

38.1K
Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.
38.1K
Flow Cytometry01:23

Flow Cytometry

16.5K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
16.5K
Flow Sheet01:17

Flow Sheet

2.9K
Flowsheets are valuable tools in nursing documentation. They enable healthcare professionals to efficiently record and monitor various patient assessments and measurements in a consolidated format.
Here's a closer look at the examples of flowsheets commonly used by nurses:
Graphic Sheet Documentation:
2.9K
Flow Table Test01:12

Flow Table Test

772
The flow table test is an established method used to assess the workability of concrete, particularly useful for evaluating highly flowable concrete mixes. This test employs an apparatus that consists of a wooden board topped with a steel plate, collectively weighing 35 pounds. The board is connected to a base via a hinge and measures 27.6 inches on each side.
Concrete is placed within a truncated cone mold that is 8 inches high with an 8-inch base diameter and a 5-inch top diameter. The...
772
Irrotational Flow01:28

Irrotational Flow

1.0K
Irrotational flow is characterized by fluid motion where particles do not rotate around their axes, resulting in zero vorticity. For a flow to be irrotational, the curl of the velocity field must be zero. This imposes specific conditions on velocity gradients. For instance, to maintain zero rotation about the z-axis, the gradient condition:
1.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a Machine Learning-Based Triage Score for Medication-Related Osteonecrosis of the Jaw in Osteoporosis Patients Undergoing Tooth Extraction.

Diagnostics (Basel, Switzerland)·2026
Same author

Efficacy of Polynucleotide for the Recovery of Depressed Lesions Following Steroid-Based Lipolysis Injections.

Clinical, cosmetic and investigational dermatology·2026
Same author

Hemolysis testing in circulatory support pumps: Standards, mock loops, and pulsatility.

The International journal of artificial organs·2026
Same author

Mapping brain function underlying naturalistic motor observation and imitation using high-density diffuse optical tomography.

Imaging neuroscience (Cambridge, Mass.)·2025
Same author

Skin-Whitening, Antiwrinkle, and Moisturizing Effects of <i>Astilboides tabularis</i> (Hemsl.) Engl. Root Extracts in Cell-Based Assays and Three-Dimensional Artificial Skin Models.

International journal of molecular sciences·2025
Same author

National and regional trend over time in the prevalence of osteonecrosis of the femoral head among military draft-eligible males in South Korea: an analysis of a nationwide database from 2013 to 2022.

European journal of orthopaedic surgery & traumatology : orthopedie traumatologie·2025

Related Experiment Video

Updated: Feb 15, 2026

Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow
10:02

Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow

Published on: April 29, 2011

16.9K

Effect of pulsatile flow perfusion on decellularization.

Sung Min Park1, Seran Yang1, Se-Min Rye1

  • 1School of Medicine, Kangwon National University, Chuncheon-si, Republic of Korea.

Biomedical Engineering Online
|February 3, 2018
PubMed
Summary
This summary is machine-generated.

Pulsatile perfusion enhances decellularization efficiency in tissue engineering scaffolds by reducing residual DNA while preserving extracellular matrix (ECM) components. This method improves scaffold quality for regenerative medicine applications.

Keywords:
DecellularizationExtracellular matrixHeartPulsatile flowScaffold

More Related Videos

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor
10:56

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

Published on: August 1, 2022

3.3K
Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
11:30

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Published on: December 6, 2012

24.5K

Related Experiment Videos

Last Updated: Feb 15, 2026

Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow
10:02

Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow

Published on: April 29, 2011

16.9K
Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor
10:56

Procurement and Perfusion-Decellularization of Porcine Vascularized Flaps in a Customized Perfusion Bioreactor

Published on: August 1, 2022

3.3K
Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion
11:30

Procedure for Decellularization of Porcine Heart by Retrograde Coronary Perfusion

Published on: December 6, 2012

24.5K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Decellularized animal organs serve as extracellular matrix (ECM) scaffolds for tissue engineering.
  • Preserving ECM components is crucial for functional scaffolds.
  • Pulsatile flow may reduce detergent exposure time during decellularization.

Purpose of the Study:

  • To investigate the effect of pulsatile flow on the decellularization process using Energy Equivalent Pressure (EEP) as a pulsatility parameter.
  • To assess the impact of pulsatile perfusion on ECM preservation and DNA removal.

Main Methods:

  • Twelve rat hearts underwent decellularization using 1% sodium dodecyl sulfate (SDS) solution for 2 hours.
  • Hearts were divided into two groups: pulsatile perfusion (n=6) and non-pulsatile perfusion (n=6).
  • Energy Equivalent Pressure (EEP) was used to quantify pulsatility.

Main Results:

  • Pulsatile perfusion significantly increased EEP and perfusion flow compared to non-pulsatile perfusion.
  • More profound decellularization was observed in the pulsatile group.
  • Residual DNA content was significantly lower in pulsatile group scaffolds, with no significant difference in collagen and glycosaminoglycan (GAG) levels.

Conclusions:

  • Pulsatile flow decellularization is more efficient than non-pulsatile flow.
  • Pulsatile flow decellularization effectively preserves essential ECM molecules.
  • This technique offers improved scaffold quality for tissue engineering applications.