Related Experiment Video
Updated: Feb 15, 2026
10:42
Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
17.9K
Immunofluorescence in oral lesions.
S J Nithya1, R Sankarnarayanan1, V T Hemalatha1
1Department of Oral Medicine and Radiology, Sree Balaji Dental College, Chennai, Tamil Nadu, India.
Journal of Oral and Maxillofacial Pathology : JOMFP
|February 3, 2018
Summary
Immunofluorescence is a key diagnostic method for identifying antigens and antibodies in autoimmune bullous diseases. This technique detects immune deposits crucial for understanding disease development.
Area of Science:
- Immunology
- Pathology
- Histology
Background:
- Immunofluorescence is an immunohistochemical technique.
- It is used to detect antigens and antibodies in tissues and serum.
- It serves as an auxiliary diagnostic tool for autoimmune bullous and inflammatory disorders.
Purpose of the Study:
- To highlight the role of immunofluorescence in diagnosing autoimmune bullous diseases.
- To explain the detection of immune deposits in disease pathogenesis.
Main Methods:
- Immunofluorescence assays.
- Immunohistochemistry.
Main Results:
- Demonstration of antigen and antibody presence.
- Detection of in situ and circulating immune deposits.
Conclusions:
- Immunofluorescence is vital for diagnosing autoimmune bullous and inflammatory disorders.
- It aids in understanding the pathogenesis of immunobullous diseases by detecting immune deposits.
Related Concept Videos
01:11Oral Cavity
3.2K
The oral cavity, or the mouth, is a complex structure in humans that plays a vital role in our day-to-day lives. Its role is not only in chewing and swallowing food; it also plays a role in speech and facial expressions.
Teeth: The teeth are the hardest structures in our bodies. Humans have two sets of teeth throughout their lifetime: deciduous (baby) teeth and permanent teeth. Each tooth consists of several parts: the crown (visible part), the root (embedded in the jaw), enamel (hard outer...
Teeth: The teeth are the hardest structures in our bodies. Humans have two sets of teeth throughout their lifetime: deciduous (baby) teeth and permanent teeth. Each tooth consists of several parts: the crown (visible part), the root (embedded in the jaw), enamel (hard outer...
3.2K
01:12Immunofluorescence Microscopy
13.9K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
13.9K
01:06Oral Hypoglycemic Agents: Glinides
700
Repaglinide (Prandin) and Nateglinide (Starlix), known as glinides, are oral insulin secretagogues that stimulate insulin release from pancreatic β cells by closing the ATP-sensitive potassium channels (KATP channel). Repaglinide controls insulin release from pancreatic β cells by managing potassium efflux. It shares two binding sites with sulfonylureas and also has a unique site, indicating overlapping mechanisms of action. With a rapid onset and a 4-7 hour duration, it effectively...
700
01:16Psychosexual Stages of Personality: Oral
3.3K
The oral stage is the initial phase of Sigmund Freud's theory of psychosexual development, occurring from birth to approximately 12 to 18 months. During this period, the infant's mouth serves as the primary source of pleasure, with actions such as sucking, chewing, biting, and drinking playing a crucial role in reducing tension. These activities are essential not only for nourishment but also for the infant's psychological and emotional satisfaction.
Weaning, typically occurring...
Weaning, typically occurring...
3.3K
01:17Oral Hypoglycemic Agents: Sulfonylureas
1.2K
Sulfonylureas are oral hypoglycemic agents utilized in treating type 2 diabetes. They are characterized by their unique sulfonylurea chemical structure. The family of sulfonylureas is divided into generations. First-generation sulfonylureas, including tolbutamide (Orinase), chlorpropamide (Diabinese), and tolazamide (Tolinase), trigger insulin release from pancreatic β cells and enhance peripheral tissues' insulin sensitivity. The second-generation members, such as glipizide...
1.2K
01:14Assessing Body Temperature - Oral
1.7K
Here are the steps to accurately measure oral temperature using an electronic thermometer:
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
1.7K