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Comparitive efficacies of a natural fixative with a conventional fixative
Nidhi Sinha1, Meghanand T Nayak1, J D Sunitha1
1Department of Oral Pathology and Microbiology, Teerthanker Mahaveer Dental College and Research Centre, Moradabad, Uttar Pradesh, India.
Journal of Oral and Maxillofacial Pathology : JOMFP
|February 3, 2018
Summary
Jaggery syrup effectively preserves tissue for histopathology, offering results comparable to formalin. This natural alternative surprisingly enhances nuclear detail, making it a viable substitute in labs.
Area of Science:
- Histopathology
- Biomedical research
Background:
- Formalin, a common tissue fixative, poses health risks, driving the search for safer alternatives.
- Honey has been identified as a potential substitute, but jaggery is explored here.
Purpose of the Study:
- To compare the tissue fixation capabilities of 30% jaggery syrup against 10% neutral-buffered formalin (NBF).
- To evaluate jaggery as a natural, safer alternative to formalin in histopathology.
Main Methods:
- Sixty-five pathological tissue specimens were divided and fixed in either 30% jaggery solution or 10% NBF for 24 hours.
- Evaluated pre- and post-fixation parameters including shrinkage, weight difference, and ease of sectioning.
- Histomorphological assessment of cellular outline, cytoplasmic and nuclear details, staining quality, and overall morphology was performed under light microscopy.
Main Results:
- Jaggery syrup demonstrated comparable tissue preservation to formalin.
- Histological evaluation revealed superior overall nuclear detail with jaggery fixation compared to formalin.
Conclusions:
- Jaggery can be successfully implemented as a routine fixative in histopathology laboratories.
- This natural substitute offers a safer alternative to formalin with comparable or improved tissue preservation.
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