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Updated: Feb 15, 2026

Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens
Published on: May 23, 2014
Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens
Amit D Gujar1, Diane D Mao1, John B Finlay1
1Department of Neurological Surgery, Washington University School of Medicine, St. Louis, MO, USA.
Abstract:
This chapter describes a method for isolation, maintenance, and propagation of primary glioblastoma (GBM) cells in adherent monolayer cultures from patient tumor specimens. This method enables the establishment of GBM cultures with stem or progenitor-like cell characteristics, including self-renewal capacity, differentiation along restricted neural lineages, and tumor-initiating potential when orthotopically injected into immunocompromised mice. This experimentally tractable model system is therefore suitable for a wide variety of analyses in vitro as well as in vivo. Key examples of biological analyses that can be performed using these cells are also described.
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