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Updated: Feb 15, 2026

Induction and Testing of Hypoxia in Cell Culture
Published on: August 12, 2011
Induction and Assessment of Hypoxia in Glioblastoma Cells In Vitro
Jean-Pierre Gagner1,2, Mirna Lechpammer3, David Zagzag4,5,6,7
1Microvascular and Molecular Neuro-Oncology Laboratory, Department of Pathology, NYU Langone Medical Center, New York, NY, USA.
Abstract:
To simulate and study the hypoxic microenvironment associated with intracerebral glioma in vivo, simple and reproducible methods are described and discussed for inducing hypoxia or chemical pseudohypoxia in glioma cell cultures and assessing their effects on the expression and nuclear translocation of hypoxia-inducible factor (HIF)-1α, a key transcriptional factor of oxygen homeostasis, by Western blot analysis and immunocytochemistry.
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