Fabricating High-Performance T₂-Weighted Contrast Agents via Adjusting Composition and Size of Nanomagnetic Iron...

Magnetic relaxation switch demonstrated that the aggregated nanomagnetic iron oxide (NMIO) nanocrystal possessed a lower T₂ value and better relaxivity compared with monodispersed NMIO nanocrystal. However, we found that NMIO nanoclusters (NMIONCs) showed a different magnetic resonance (MR) imaging property in comparison with NMIO nanocrystals. Herein, three types of NMIONCs were used to explore the effects of size and compositions on the variations of magnetism and MR contrast ability. It was found that the transverse relaxation rate (r₂) of NMIONCs depended on the contact area between particles and water molecules. The smaller size and higher solubility could carry out higher contact area between NMIONCs and water molecules. Therefore, the monodispersed NMIONC showed a better T₂ contrast ability in comparison with the aggregated NMIONC. In addition, for NMIONCs with the same composition, the magnetism and contrast ability gradually increased with the particle size decreasing. In vivo, NMIONCs that possessed the best solubility and the smallest size showed the most effective MR contrast effect for the liver region of mice. As a result, the size and composition of NMIONCs played important roles for enhancing contrast behavior. This study provides a new idea to develop high-performance T₂ contrast agents by modulating the size and composition of particles.