Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

16.9K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.9K
Protein Complex Assembly02:41

Protein Complex Assembly

2.6K
2.6K
Spindle Assembly02:50

Spindle Assembly

4.4K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
4.4K
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

3.7K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
3.7K
Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

28.0K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
28.0K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

21.1K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
21.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aptamer-Based Delivery Systems for VEGF and NGF Modulation in Ocular Therapies.

Advanced healthcare materials·2026
Same author

Muscle-fiber-inspired nanofibrillar microbundles induce myogenic differentiation in human adipose-derived stem cells.

Bioactive materials·2026
Same author

Phosphate- and pH-dependent self-assembly of recombinant spider silk proteins.

Protein science : a publication of the Protein Society·2026
Same author

Biocompatibility of Hydrogels for Glomerular 3D Co-Culture: A Comparative Analysis.

Macromolecular bioscience·2026
Same author

Biofabrication of Endothelialized, Intrinsically Vascularized 3D-Printed Recombinant Spider Silk Scaffolds.

Advanced healthcare materials·2026
Same author

Directed Functionalization of Recombinant Spider Silk Nonwoven Membranes with Antibodies Using Non-Canonical Amino Acids.

Advanced materials (Deerfield Beach, Fla.)·2026

Related Experiment Video

Updated: Feb 15, 2026

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning
12:07

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Published on: April 16, 2018

14.1K

Silk nanofibril self-assembly versus electrospinning.

Martin Humenik1, Gregor Lang1, Thomas Scheibel1,2

  • 1Biomaterials, Faculty of Engineering Science, University of Bayreuth, Bayreuth, Germany.

Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
|February 3, 2018
PubMed
Summary
This summary is machine-generated.

Silk proteins self-assemble into hierarchical nanostructures and can be processed into nanofibers. These advanced biomaterials offer diverse morphologies for various applications.

Keywords:
electrospinningnanofibersnanofibrilsrecombinant proteinsself-assemblysilk

More Related Videos

High-resolution Patterning Using Two Modes of Electrohydrodynamic Jet: Drop on Demand and Near-field Electrospinning
09:16

High-resolution Patterning Using Two Modes of Electrohydrodynamic Jet: Drop on Demand and Near-field Electrospinning

Published on: July 10, 2018

10.3K
Designing Silk-silk Protein Alloy Materials for Biomedical Applications
11:14

Designing Silk-silk Protein Alloy Materials for Biomedical Applications

Published on: August 13, 2014

18.9K

Related Experiment Videos

Last Updated: Feb 15, 2026

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning
12:07

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Published on: April 16, 2018

14.1K
High-resolution Patterning Using Two Modes of Electrohydrodynamic Jet: Drop on Demand and Near-field Electrospinning
09:16

High-resolution Patterning Using Two Modes of Electrohydrodynamic Jet: Drop on Demand and Near-field Electrospinning

Published on: July 10, 2018

10.3K
Designing Silk-silk Protein Alloy Materials for Biomedical Applications
11:14

Designing Silk-silk Protein Alloy Materials for Biomedical Applications

Published on: August 13, 2014

18.9K

Area of Science:

  • * Biology-Inspired Nanomaterials
  • * Nanotechnology Approaches to Biology

Background:

  • * Natural silk fibers possess highly optimized mechanical properties due to hierarchical structures.
  • * Biotechnological production and advanced processing unlock silk proteins' potential beyond mechanical strength.
  • * Diverse silk protein morphologies, including nanoscale fibrils and fibers, are suitable for medical and technical applications.

Purpose of the Study:

  • * To introduce the self-assembly of silk proteins into hierarchically organized supramolecular nanofibrils.
  • * To present electrospinning as a technique for producing silk nanofibers and nanofibrous mats.
  • * To explore a broad range of silk-based materials for self-assembly and electrospinning.

Main Methods:

  • * Investigating the natural self-assembly mechanism of silk proteins during natural spinning.
  • * Utilizing self-assembly to create silk protein-based supramolecular nanofibrils.
  • * Employing electrospinning to fabricate silk nanofibers and nanofibrous mats.
  • * Developing diverse silk-based dopes, including natural, reconstituted, recombinant, and hybrid proteins.

Main Results:

  • * Demonstrated the formation of hierarchically organized silk protein structures via self-assembly.
  • * Successfully produced silk nanofibers and nanofibrous mats using electrospinning.
  • * Characterized a wide array of silk-based dopes for various fabrication techniques.
  • * Highlighted the advantages and disadvantages of different silk production and processing routes.

Conclusions:

  • * Silk proteins can be engineered into advanced nanostructured scaffolds with tunable properties.
  • * Both self-assembly and electrospinning are viable methods for producing silk-based nanomaterials.
  • * Silk-derived nanostructures offer promising biocompatibility, robustness, and high surface-to-volume ratios for applications.