Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Formation of Complex Ions03:45

Formation of Complex Ions

26.3K
A type of Lewis acid-base chemistry involves the formation of a complex ion (or a coordination complex) comprising a central atom, typically a transition metal cation, surrounded by ions or molecules called ligands. These ligands can be neutral molecules like H2O or NH3, or ions such as CN− or OH−. Often, the ligands act as Lewis bases, donating a pair of electrons to the central atom. These types of Lewis acid-base reactions are examples of a broad subdiscipline called coordination...
26.3K
Hydrogen Bonds00:26

Hydrogen Bonds

134.8K
Hydrogen bonds are weak attractions between atoms that have formed other chemical bonds. One of these atoms is electronegative, like oxygen, and has a partial negative charge. The other is a hydrogen atom that has bonded with another electronegative atom and has a partial positive charge.
Hydrogen Bonds Control the World!
Because hydrogen has very weak electronegativity when it binds with a strongly electronegative atom, such as oxygen or nitrogen, electrons in the bond are unequally shared....
134.8K
Hydrogen Bonds01:04

Hydrogen Bonds

15.2K
A hydrogen bond is formed when a weakly positive hydrogen atom already bonded to one electronegative atom (for example, the oxygen in the water molecule) is attracted to another electronegative atom from another polar molecule, such as water (H2O), hydrogen fluoride (HF), or ammonia (NH3). The huge electronegativity difference between the H atom (2.1) and the atom to which it is bonded (4.0 for an F atom, 3.5 for an O atom, or 3.0 for an N atom), combined with the very small size of an H atom...
15.2K
Preparation and Reactions of Sulfides02:26

Preparation and Reactions of Sulfides

5.8K
Sulfides are the sulfur analog of ethers, just as thiols are the sulfur analog of alcohol. Like ethers, sulfides also consist of two hydrocarbon groups bonded to the central sulfur atom. Depending upon the type of groups present, sulfides can be symmetrical or asymmetrical. Symmetrical sulfides can be prepared via an SN2 reaction between 2 equivalents of an alkyl halide and one equivalent of sodium sulfide.
5.8K
Structure and Nomenclature of Thiols and Sulfides02:17

Structure and Nomenclature of Thiols and Sulfides

5.8K
Thiols and sulfides are sulfur analogs of alcohols and ethers, respectively, where the sulfur atom takes the place of the oxygen atom. Thus, thiols are generally represented as RSH, where R is an alkyl substituent and —SH is the functional group. On the other hand, in sulfides, the central sulfur atom is bonded to two hydrocarbon groups on either side. Depending upon the type of group, sulfides can be either symmetrical or asymmetrical. Both thiols and sulfides display a bent geometry,...
5.8K
Reduction of Alkenes: Catalytic Hydrogenation02:13

Reduction of Alkenes: Catalytic Hydrogenation

14.3K
Alkenes undergo reduction by the addition of molecular hydrogen to give alkanes. Because the process generally occurs in the presence of a transition-metal catalyst, the reaction is called catalytic hydrogenation.
Metals like palladium, platinum, and nickel are commonly used in their solid forms — fine powder on an inert surface. As these catalysts remain insoluble in the reaction mixture, they are referred to as heterogeneous catalysts.
The hydrogenation process takes place on the...
14.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Macroscopic Experiment Study and Microscopic Molecular Simulation of Adsorption Interaction between Heavy Asphaltene and Shale Illite.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Intercalating cerium into layered double hydroxide as a promising reactive oxygen species scavenger for inflammation treatment.

Journal of materials chemistry. B·2025
Same author

A cobalt(III) complex as a dual-mode probe for the detection of sodium dithionite <i>via</i> MRI and fluorescence.

Analytical methods : advancing methods and applications·2025
Same author

Self-Immolative Cationic Iridium(III) Complex-Encapsulated Nanoprodrug for Enhanced Chemo-Photodynamic Synergistic Therapy of Melanomas.

Journal of the American Chemical Society·2025
Same author

Lysozyme-coated nanoparticles for active uptake and delivery of synthetic RNA and plasmid-encoded genes in plants.

Nature plants·2025
Same author

Bioorthogonally activated probes for precise fluorescence imaging.

Chemical Society reviews·2024
Same journal

Machine-Learning-Enabled Rapid Evolution of Photoenzymes for the Asymmetric Synthesis of gem-Difluorophosphonates.

Angewandte Chemie (International ed. in English)·2026
Same journal

Sequential H<sub>2</sub>S-Triggered Redox Relay Nanoprobes for Self-Sustained Chem-Illuminating Cascade Photodynamic Therapy.

Angewandte Chemie (International ed. in English)·2026
Same journal

Quantitative Active Hydrogen Modulation via Mastering Interfacial Water Over Single Rare Earth Atom on Copper for NO<sub>3</sub> <sup>-</sup>-to-NH<sub>3</sub> Electroreduction.

Angewandte Chemie (International ed. in English)·2026
Same journal

Unveiling the Role of Hydroxyls on Catalyst Surface in CO<sub>2</sub> Hydrogenation Reaction.

Angewandte Chemie (International ed. in English)·2026
Same journal

Strain-Release Pentafluorosulfanylation of Carbonyl-Containing Disubstituted Bicyclobutanes: A Fortuitous Path to SF<sub>5</sub>-Containing Oxa[2.1.1]bicyclohexanes.

Angewandte Chemie (International ed. in English)·2026
Same journal

Quantum Spin-1/2 Rings Built From [2]Triangulene Molecular Units.

Angewandte Chemie (International ed. in English)·2026
See all related articles

Related Experiment Video

Updated: Feb 15, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
03:55

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

Published on: June 27, 2022

4.2K

Quantitative Monitoring and Visualization of Hydrogen Sulfide In Vivo Using a Luminescent Probe Based on a

Zhongbo Du1, Bo Song1, Wenzhu Zhang1

  • 1State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian, 116024, China.

Angewandte Chemie (International Ed. in English)
|February 3, 2018
PubMed
Summary
This summary is machine-generated.

A new ruthenium-based probe, Ru-MDB, enables sensitive detection of hydrogen sulfide (H₂S) in biological samples. This probe allows for real-time imaging of H₂S in live organisms, advancing our understanding of its physiological roles.

Keywords:
analytical methodshydrogen sulfidein vivo sensingruthenium complexeszebrafish models

More Related Videos

The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes
10:51

The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes

Published on: April 10, 2015

12.8K
Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor
07:12

Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor

Published on: October 26, 2017

8.2K

Related Experiment Videos

Last Updated: Feb 15, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
03:55

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

Published on: June 27, 2022

4.2K
The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes
10:51

The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes

Published on: April 10, 2015

12.8K
Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor
07:12

Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor

Published on: October 26, 2017

8.2K

Area of Science:

  • Bioanalytical Chemistry
  • Chemical Biology
  • Inorganic Chemistry

Background:

  • Hydrogen sulfide (H₂S) is a crucial gasotransmitter involved in various physiological and pathological processes.
  • Accurate monitoring of H₂S in live biological systems is essential for understanding its functions.
  • Existing methods for H₂S detection face challenges in complex biological environments.

Purpose of the Study:

  • To develop a novel, sensitive, and specific bioanalytical probe for monitoring H₂S in live organisms.
  • To utilize a ruthenium(II)-complex-based luminescence probe with a unique H₂S-responsive masking moiety.
  • To enable quantitative detection and imaging of H₂S in diverse biological matrices and live subjects.

Main Methods:

  • Development of a red-emitting ruthenium(II)-complex probe (Ru-MDB) incorporating an H₂S-cleavable masking group.
  • Utilizing a luminescence "off-on" response triggered by H₂S-mediated cleavage of the masking moiety.
  • Employing time-gated luminescence for quantitative H₂S detection in complex samples like human sera and zebrafish organs.
  • In vivo imaging and flow cytometry analysis in various model organisms (Daphnia magna, zebrafish, mice) and cell types.

Main Results:

  • The Ru-MDB probe demonstrated a luminescence "off-on" response upon reaction with H₂S.
  • Long-lived emissions allowed sensitive and specific quantitative detection of H₂S, even in autofluorescence-rich samples.
  • The probe exhibited low cytotoxicity, enabling live-cell imaging and analysis of lysosomal H₂S generation.
  • Successful in vivo imaging of H₂S was achieved in multiple organisms, including zebrafish embryos, adult zebrafish, and mice.

Conclusions:

  • Ru-MDB is a highly effective red-emitting luminescence probe for sensitive and specific H₂S detection.
  • The probe's properties facilitate quantitative analysis and imaging of H₂S in complex biological systems and live organisms.
  • This development provides a valuable tool for advancing research into the physiological and pathological roles of H₂S.