Related Experiment Video
Updated: Feb 15, 2026

Spectrophotometric Determination of Phycobiliprotein Content in Cyanobacterium Synechocystis
Published on: September 11, 2018
Analysis of octyl methoxycinnamate in sunscreen products by a validated UV-spectrophotometric method
Abstract:
An inexpensive, rapid method for the determination of octyl methoxycinnamate (OMC) in sunscreen products using ultraviolet-spectrophotometry has been developed and validated according to International Council for Harmonisation and Association of Official Analytical Chemists guidelines. Methanol was the optimal solvent used with a linearity range of 4–12 µg\/ml (r = 0.999) being obtainable. The accuracy of the method is highlighted by the % recovery (98.23–98.50) and relative standard deviation (%RSD, 0.12), and it is widely applicable to prototype products composed of oil in water, and water in oil emulsions. Mineral oils containing low, intermediate, and high OMC levels (1%, 4%, and 7.5%) gave recovery percentages of 99.76–100.76 with %RSD of 0.02–0.28. In addition, this method is repeatable and affords a high degree of precision (%RSD = 0.12 and 0) with 96.08–99.27% recovery. The method is suitable for quality assurance of suncare product formulations, and could be applicable to product development and validation.
Related Concept Videos
Reliability and Validity
UV–Vis Spectrum
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar...
Data Validation
Key parameters for method validation include:
Data Validation
Nursing assessment guides are generally based on holistic models rather than medical...
UV–Vis Spectrometers
Clinically Relevant Drug Product Specifications: Methods of Establishment

