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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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RNA Splicing01:32

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Exon Recombination02:32

Exon Recombination

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The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
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Null and Alternative Hypotheses01:16

Null and Alternative Hypotheses

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The actual hypothesis testing begins by considering two hypotheses. They are termed  the null hypothesis and the alternative hypothesis. These hypotheses contain opposing viewpoints.
The null hypothesis, denoted by H0 is a statement of no difference between the variables—they are not related. This can often be considered the status quo. As  a result if you cannot accept the null, it requires some action.
The alternative hypothesis, denoted by H1 or Ha, is a claim about the...
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Chromatin Structure and RNA Splicing02:41

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Related Experiment Video

Updated: Feb 15, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

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Universal Alternative Splicing of Noncoding Exons.

Ira W Deveson1, Marion E Brunck2, James Blackburn3

  • 1Garvan Institute of Medical Research, Sydney, NSW, Australia; School of Biotechnology and Biomolecular Sciences, Faculty of Science, University of New South Wales, Sydney, NSW, Australia.

Cell Systems
|February 4, 2018
PubMed
Summary
This summary is machine-generated.

Researchers explored the human transcriptome using advanced RNA sequencing (RNA-seq) to reveal novel insights into gene expression and alternative splicing of noncoding exons.

Keywords:
RNA CaptureSeqRNA sequencingalternative splicinglncRNAmammalian transcriptomenoncoding RNA

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Related Experiment Videos

Last Updated: Feb 15, 2026

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Area of Science:

  • Genomics
  • Molecular Biology
  • Transcriptomics

Background:

  • The human transcriptome is vast and dynamic, posing challenges for comprehensive analysis.
  • Current RNA sequencing (RNA-seq) methods exhibit expression-dependent biases, limiting the detection of low-abundance transcripts.

Purpose of the Study:

  • To characterize the human transcriptome at unprecedented resolution, focusing on a single human chromosome (Hsa21).
  • To investigate the nature of noncoding exons and their role in transcript diversity.

Main Methods:

  • Targeted single-molecule and short-read RNA sequencing (RNA-seq) of human chromosome 21 (Hsa21).
  • Comparative analysis of syntenic regions in the mouse genome.
  • Assessment of splicing profile conservation across species.

Main Results:

  • Identified a fundamental distinction between protein-coding and noncoding gene content.
  • Demonstrated that nearly all noncoding exons undergo alternative splicing, generating numerous isoforms.
  • Showcased conservation of human splicing patterns in mouse cells, suggesting a conserved splicing code.

Conclusions:

  • Noncoding exons are functionally modular and contribute to a vast repertoire of regulatory RNAs.
  • Alternative splicing of noncoding exons provides a rich reservoir for gene evolution.
  • A conserved splicing code likely governs transcript diversity across species.