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Characterization of bone morphology in CCN5/WISP5 knockout mice
Jie Jiang1, Gexin Zhao2,3, Karen M Lyons4,5
1Department of Pediatrics, Rush University Medical Center, Chicago, IL, USA.
Journal of Cell Communication and Signaling
|February 4, 2018
Summary
CCN5 (Cell-Cell Nucleus 5) protein is not essential for normal bone formation in mice. Studies using Ccn5 knockout mice show no changes in bone density, but suggest potential roles in other processes.
Area of Science:
- Biochemistry
- Genetics
- Molecular Biology
Background:
- CCN5/WISP2 is a matricellular protein lacking a C-terminal domain.
- Previous in vitro studies suggest CCN5 influences cell proliferation and differentiation.
- The in vivo function of CCN5 remains largely uncharacterized.
Purpose of the Study:
- To investigate the in vivo role of CCN5 in adult bone homeostasis.
- To characterize the phenotype of Ccn5 knockout mice.
Main Methods:
- Generated Ccn5 knockout mice (Ccn5 LacZ/LacZ) using KOMP ES cells.
- Analyzed lacZ expression in adult bone tissues.
- Performed Micro-CT analysis to assess bone mineral density (BMD) and bone tissue volume (BV/TV).
Main Results:
- Ccn5 LacZ/LacZ mice were viable and phenotypically normal.
- LacZ expression was detected in the periosteum but not in trabecular bone or bone marrow.
- No significant alterations in BMD or BV/TV were observed in knockout mice.
Conclusions:
- CCN5 is not required for normal adult bone formation.
- These findings do not exclude a role for CCN5 in bone mechanotransduction or repair.
- Ccn5 knockout mice provide a valuable tool for studying CCN5 function in various tissues.
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