[Assay of plasma dehydroepiandrosterone sulfate by gas chromatography with electron-capture detection]

A sensitive and specific method for the determination of plasma S-DHA by gas chromatography (GC), with fused silica capillary column (stationary phase SE 54) and electron capture, after solvolysis of the sample and purification by high performance liquid chromatography (HPLC) is described. The interest of the new internal standard (5 alpha androst-9(11)-ene-3 beta ol-17-one) for the determination of the DHA by GC is shown. The analytical characteristics of this method as well as a comparative study of the values obtained by this method and by radioimmuno-assay are described.