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The effects of different preservation methods on ide (Leuciscus idus) sperm and the longevity of sperm movement
G Bernáth1, Zs Csenki1, Z Bokor1
1Department of Aquaculture, Szent István University, Páter Károly u. 1., H-2100 Gödöllő/Tópart u 5309/8 Hrsz, H-2484 Agárd (Gárdony), Hungary.
Abstract:
The present study investigated the effects of chilled storage and cryopreservation on ide sperm motility and fertilizing capacity alongside the longevity of sperm movement. The parameters of motility (progressive motility-pMOT, curvilinear velocity-VCL and straightness-STR) have been recorded during 48 h of chilled storage (4 °C) at 24-h intervals. The longevity of sperm movement was measured following activation for up to 120 s (in a range at 10-120 s) in freshly stripped and thawed sperm. A formerly established cryopreservation method was tested on ide sperm where motility parameters, hatching rate and larval malformation (according to 7 category groups) were investigated. Significant decrement of pMOT has already been observed after 24 h (6 ± 5%) compared to the freshly stripped sperm (49 ± 22%). pMOT and STR showed no significant changes for up to 120 s following activation in fresh sperm, whereas VCL showed significant difference between 10 (51 ± 11 μm/s), 90 (33 ± 3 μm/s) and 120 (31 ± 4 μm/s) seconds as well as between 20 (48 ± 12 μm/s), and 120 s. No negative effect of cryopreservation was recorded on pMOT (fresh: 49 ± 19%, cryopreserved: 22 ± 22%), VCL (fresh: 45 ± 9 μm/s and cryopreserved: 57 ± 5 μm/s), STR (fresh: 81 ± 3% and cryopreserved: 92 ± 1%) hatching rate (fresh: 22 ± 15%, cryopreserved: 33 ± 18%) or larval malformation (fresh: 12 ± 4%, cryopreserved: 12 ± 4%). No significant correlation was found between the three motility parameters and hatching rate. Cryopreservation had no effect on hatching and the prevalence of larval deformity. Furthermore craniofacial and eye deformities were characteristic in the group originating from fertilization with cryopreserved sperm, while edemas (pericardial, yolk) occurred more frequently in the control. The formerly developed cryopreservation protocol (method for cyprinids) was applicable to ide sperm.
Insights
Chilled storage significantly reduced ide sperm motility within 24 hours. However, cryopreservation maintained sperm quality and hatching rates, proving effective for ide sperm preservation.
Area of Science:
- Aquaculture and Reproductive Biology
- Sperm Cryopreservation
- Fish Gamete Preservation
Background:
- Effective sperm preservation is crucial for aquaculture and genetic resource management.
- Understanding the impact of storage methods on sperm quality is essential for successful fertilization outcomes.
- Ide sperm motility and fertilizing capacity are key indicators of reproductive success.
Purpose of the Study:
- To investigate the effects of chilled storage and cryopreservation on ide sperm motility and fertilizing capacity.
- To assess the longevity of sperm movement under different preservation conditions.
- To evaluate the applicability of a previously established cryopreservation protocol for ide sperm.
Main Methods:
- Sperm motility parameters (progressive motility, curvilinear velocity, straightness) were recorded during 48-hour chilled storage at 4°C.
- Sperm movement longevity was measured post-activation for up to 120 seconds.
- A cryopreservation method was applied to ide sperm, followed by assessment of motility, hatching rate, and larval malformation.
Main Results:
- Chilled storage led to a significant decrease in progressive motility after 24 hours.
- Cryopreservation did not negatively impact sperm motility parameters, hatching rate, or larval malformation.
- Specific larval deformities (craniofacial, eye) were observed with cryopreserved sperm, while edema was more frequent in the control group.
Conclusions:
- Cryopreservation is a viable method for preserving ide sperm quality and fertilizing capacity.
- Chilled storage is less effective for long-term ide sperm preservation compared to cryopreservation.
- The tested cryopreservation protocol is applicable to ide sperm, offering potential for genetic resource management in aquaculture.
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