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Updated: Feb 15, 2026
10:27
A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
Published on: April 20, 2018
11.4K
Transcriptome analysis and development of simple sequence repeat (SSR) markers in Zingiber striolatum Diels
Kuanping Deng1, Renju Deng2, Jianxin Fan3
1Zunyi Academy of Agricultural Sciences, Zunyi, 563100 Guizhou China.
Summary
High-throughput sequencing of Zingiber striolatum Diels identified thousands of simple sequence repeat (SSR) markers. These SSR markers are valuable resources for genetic diversity analysis and breeding improvement.
Area of Science:
- Plant genomics
- Molecular biology
- Biotechnology
Background:
- Zingiber striolatum Diels is an important medicinal plant.
- Comprehensive genomic resources are needed for its genetic improvement.
Purpose of the Study:
- To perform high-throughput transcriptome sequencing of Zingiber striolatum Diels.
- To identify simple sequence repeat (SSR) markers for genetic studies and breeding.
Main Methods:
- Illumina-based paired-end sequencing of multiple Zingiber striolatum Diels tissues.
- De novo assembly of transcriptome data.
- Identification and validation of simple sequence repeat (SSR) markers using polymerase chain reaction (PCR).
Main Results:
- Generated over 130 million sequencing reads, assembling into 112,107 unigenes.
- Identified 51,804 genes with similarity to known sequences.
- Discovered 8,384 simple sequence repeat (SSR) markers, with 25 primer pairs validated for polymorphism.
Conclusions:
- The study provides a valuable set of unigene sequences and SSR markers for Zingiber striolatum Diels.
- Identified SSR markers can be utilized for genetic diversity analysis, linkage mapping, and variety improvement in Zingiber striolatum Diels breeding programs.
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