Modulation of mitochondrial ATPase sensitivity to inhibitors and stimulators by diet-induced changes in membrane...

Weanling rats were fed diets differing in fatty acid composition to determine if changes induced in cardiac mitochondrial membrane structural components alter the sensitivity of mitochondrial ATPase to inhibition by oligomycin and stimulation by 2,4-dinitrophenol. Mitochondrial ATPase assayed in situ within the mitochodrial membrane isolated from animals fed diets higher in fatty acids of longer chain length, exhibited greater oligomycin sensitivity and lower 2,4-dinitrophenol-induced stimulation. Concomitant diet-induced changes occur in the fatty acid composition of phosphatidylcholine, phosphatidylethanolamine and cardiolipin, increasing overall length of fatty-acyl tails in the membrane phospholipids. Diet fat mediated alterations in oligomycin sensitivity of mitochondrial ATPase and membrane fatty acid chain length suggest that in vivo changes in thickness of the lipid bilayer may alter mitochondrial ATPase functions. The present study extends the concept that dietary fat affects mitochondrial membrane structure and function by demonstrating that the membrane-dependent sensitivity of mitochondrial ATPase to inhibitors and stimulators may be modulated by dietary fat.