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Related Concept Videos

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Self-Presentation

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Self-presentation is a fundamental aspect of social interaction, shaping both how others perceive individuals and how they view themselves. This dynamic process influences behaviors in various social settings, often leading people to adjust their appearance, speech, and demeanor to align with their desired identity. While self-presentation can be deliberate or unconscious, it plays a critical role in interpersonal relationships and self-perception.Forms of Self-PresentationSelf-presentation can...
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People can go to great lengths to protect their self-image and present themselves in ways that they want others to see them. Sociologist Erving Goffman presented the idea that a person is like an actor on a stage. Calling his theory dramaturgy, Goffman believed that we use “impression management” to present ourselves to others as we hope to be perceived. Each situation is a new scene, and individuals perform different roles depending on who is present (Goffman, 1959). Think about...
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Strategic self-presentation refers to individuals' intentional efforts to influence how others perceive them. This process is employed in various social and professional settings, such as job interviews, dating, politics, and legal contexts, where individuals seek to shape impressions to gain social or material advantages. While people generally present themselves in ways that align with their authentic characteristics, external factors, such as cognitive load, can hinder their ability to...
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Processes of Self-Presentation01:29

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Effective self-presentation is a central component of social interaction and identity construction. It relies on the dynamic processes of defining the situation and engaging in self-disclosure. These mechanisms help individuals navigate social context expectations and manage how others perceive them, fostering mutual understanding and relationship development.Defining the SituationSocial situations are shaped by collectively understood frames—a set of widely understood rules or...
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Yeast Signaling01:28

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Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
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Strategies of Self-Presentation II: Self-Verification01:17

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Self-verification is a fundamental psychological drive wherein individuals seek affirmation of their self-concept from others, striving for consistency between their internal self-view and external perceptions. This drive operates even when the self-concept is negative, influencing interpersonal behavior and feedback preferences in complex and often counterintuitive ways. Unlike the self-enhancement motive, which seeks positive evaluations, self-verification prioritizes coherence and...
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Establishment of a yeast-based VLP platform for antigen presentation.

David Wetzel1,2, Theresa Rolf3, Manfred Suckow3

  • 1ARTES Biotechnology GmbH, Elisabeth-Selbert-Straße 9, 40764, Langenfeld, Germany. d.wetzel@artes-biotechnology.com.

Microbial Cell Factories
|February 7, 2018
PubMed
Summary

A novel yeast-based platform efficiently produces chimeric virus-like particles (VLPs) displaying foreign antigens. This cost-effective system enables the development of advanced vaccines by presenting diverse, large antigens on stable VLPs.

Keywords:
Animal infectious diseasesAntibiotic-freeAntigen presentationChimeric virus-like particlesDHBVHansenula polymorphaPichia angustaVirus-like particles

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Area of Science:

  • Biotechnology
  • Vaccine Development
  • Protein Engineering

Background:

  • Virus-like particles (VLPs) are valuable for vaccine and drug delivery systems.
  • Challenges include cost-effective production and stable incorporation of large foreign antigens into VLP scaffolds.

Purpose of the Study:

  • To establish a versatile and cost-effective platform for chimeric VLP development.
  • To utilize the duck hepatitis B virus small surface protein (dS) as a VLP scaffold.
  • To express large foreign viral antigens in yeast for VLP incorporation.

Main Methods:

  • Genetic fusion of eight large viral antigens (up to 412 amino acids) to the dS protein.
  • Heterologous expression in the yeast *Hansenula polymorpha*.
  • Purification of chimeric VLPs using established methods.

Main Results:

  • Successful expression of dS-fusion proteins and generation of chimeric VLPs.
  • Chimeric VLPs achieved up to 95% purity, containing 33% fusion protein.
  • Yields of approximately 40 mg of chimeric VLP per 100 g dry cell weight were obtained.
  • Demonstrated stability of purified VLPs for 6 months at 4°C.

Conclusions:

  • The dS-based VLP scaffold effectively incorporates diverse, large foreign protein sequences.
  • The platform is suitable for displaying immunogenic antigens from various pathogens.
  • Yeast-based production offers a cost-effective alternative for VLP development, applicable beyond small-scale research.