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Updated: Feb 14, 2026

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Comparative Assessment of Conventional Papanicolaou and Modified Ultrafast Papanicolaou Stains in Fine Needle

P Arul1, S Eniya1, Magesh Pushparaj1

  • 1Department of Pathology, Dhanalakshmi Srinivasan Medical College and Hospital, Siruvachur, Perambalur, Tamil Nadu, India.

Journal of Cytology
|February 7, 2018
PubMed
Abstract

Insights

The modified ultrafast Papanicolaou (MUFP) stain is a rapid and reliable tool for diagnosing fine needle aspiration (FNA) and body fluid samples. This method offers accurate results, enabling immediate diagnoses with high-quality staining in most cases.

Area of Science:

  • Cytopathology
  • Histopathology
  • Diagnostic Stains

Background:

  • Conventional Papanicolaou (Pap) stain has many modifications to improve reporting turnaround time.
  • The modified ultrafast Pap (MUFP) stain is a popular modification, but its use in fine needle aspiration (FNA) and body fluid evaluation is limited.

Purpose of the Study:

  • To assess the effectiveness of MUFP stain for various FNA samples and body fluids.
  • To compare MUFP stain findings with conventional Pap stain results.

Main Methods:

  • A cross-sectional study involving 301 samples (255 FNA, 46 body fluids).
  • Preparation of smears stained with both conventional Pap and MUFP stains.
  • Calculation of concordance rates and assessment of MUFP stain quality using a Quality Index (QI) and categorization (excellent, good, fair).

Main Results:

  • 100% concordance rate between MUFP stain and conventional Pap stain.
  • High Quality Index (QI) scores for MUFP stain across various sample types (0.9-1.0).
  • Excellent or good stain quality enabling easy diagnosis in 77.8% of cases; fair quality in 22.2% required more effort.

Conclusions:

  • MUFP stain is a rapid, reliable diagnostic tool for FNA and body fluid samples, suitable for routine use.
  • It facilitates immediate diagnoses, though caution is advised to prevent over/under-diagnosis in some cases.
  • The study supports the regular application of MUFP stain in cytopathology laboratories.
Keywords:
Body fluidsconventional Papanicolaou stainfine needle aspiration cytologymodified ultrafast Papanicolaou stain

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