Related Experiment Video

Updated: Feb 14, 2026

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
11:29

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

Published on: December 3, 2011

12.7K

In vitro cost-effective methods to detect carbapenemases in Enterobacteriaceae

Varsha Gupta1, Ranu Soni1, Neha Jain1

  • 1Department of Microbiology, Government Medical College Hospital, Chandigarh, India.

Journal of Laboratory Physicians
|February 7, 2018
PubMed

Insights

Detecting carbapenemase-producing organisms is crucial for effective treatment. An in-house modified Carba NP (MCNP) test and the carbapenem inactivation method (CIM) test are reliable and cost-effective detection strategies.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Clinical Diagnostics

Background:

  • Carbapenemase-producing organisms (CPOs) pose a significant threat due to limited treatment options.
  • Key CPO types include Klebsiella pneumoniae carbapenemase (Ambler class A), VIM, IMP, NDM (Ambler class B), and OXA-48 (Ambler class D).

Purpose of the Study:

  • To evaluate the efficiency of different methods for detecting carbapenemase production.
  • To identify reliable and cost-effective diagnostic strategies for CPOs.

Main Methods:

  • Evaluation of four detection methods: Carba NP test, modified Carba NP (MCNP) test, carbapenem inactivation method (CIM) test, and Rapidec Carba NP kit.
  • Assessment of an in-house MCNP test using a single protocol.
  • Evaluation of CIM using routine antibiotic discs.

Main Results:

  • The in-house MCNP test demonstrated reliable carbapenemase detection.
  • The CIM test using routine antibiotic discs also yielded good results.
  • Both MCNP and CIM tests were found to be cost-effective.

Conclusions:

  • The modified Carba NP (MCNP) test and carbapenem inactivation method (CIM) are effective and economical approaches for detecting carbapenemase producers.
  • These validated methods can aid in selecting appropriate therapeutic interventions for CPO infections.
Keywords:
CarbapenemasesEnterobacteriaceaeimipenem

More Related Videos

Efficient and Cost Effective Electroporation Method to Study Primary Cilium-Dependent Signaling Pathways in the Granule Cell Precursor04:06

Efficient and Cost Effective Electroporation Method to Study Primary Cilium-Dependent Signaling Pathways in the Granule Cell Precursor

Published on: November 30, 2021

2.7K
A Cost Effective and Adaptable Scratch Migration Assay08:59

A Cost Effective and Adaptable Scratch Migration Assay

Published on: June 30, 2020

6.3K

Related Concept Videos

Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
669
Precipitation Titration: Endpoint Detection Methods01:19

Precipitation Titration: Endpoint Detection Methods

In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
In the Volhard method, a standard excess of AgNO3 is first added to the...
6.1K
Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
646
In Vitro Drug Dissolution: Alternative Methods01:17

In Vitro Drug Dissolution: Alternative Methods

Alternative drug dissolution methods include the rotating bottle, intrinsic dissolution test, peristalsis, and the Franz diffusion cell method. The rotating bottle method involves meticulously rotating tightly capped controlled-release beads in a temperature-controlled bath. Periodic decanting of samples allows for residue assay, followed by refilling with fresh medium and testing at various pH levels to emulate the gastrointestinal tract conditions.In contrast, the intrinsic dissolution test...
260
Buffer Effectiveness02:19

Buffer Effectiveness

Buffer solutions do not have an unlimited capacity to keep the pH relatively constant . Instead, the ability of a buffer solution to resist changes in pH relies on the presence of appreciable amounts of its conjugate weak acid-base pair. When enough strong acid or base is added to substantially lower the concentration of either member of the buffer pair, the buffering action within the solution is compromised.
The buffer capacity is the amount of acid or base that can be added to a given volume...
55.6K
Biological Effects of Radiation02:59

Biological Effects of Radiation

All radioactive nuclides emit high-energy particles or electromagnetic waves. When this radiation encounters living cells, it can cause heating, break chemical bonds, or ionize molecules. The most serious biological damage results when these radioactive emissions fragment or ionize molecules. For example, α and β particles emitted from nuclear decay reactions possess much higher energies than ordinary chemical bond energies. When these particles strike and penetrate matter, they...
18.1K