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Related Concept Videos

Circles01:18

Circles

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A circle in the coordinate plane is defined as the set of all points that lie at a constant distance, known as the radius, from a fixed point called the center. This relationship is captured using the distance formula. For a point (x, y) on the circle and a center (h, k), the distance between them equals the radius r. By squaring both sides of the distance formula, the equation of the circle is written in standard form:Constructing the Equation from Geometric InformationIf the center and the...
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Rolling Without Slipping01:09

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People have observed the rolling motion without slipping ever since the invention of the wheel. For example, one can look at the interaction between a car's tires and the surface of the road. If the driver presses the accelerator to the floor so that the tires spin without the car moving forward, there must be kinetic friction between the wheels and the road's surface. If the driver slowly presses the accelerator, causing the car to move forward, the tires roll without slipping. It is...
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Rolling With Slipping01:14

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Rolling with slipping is a physical phenomenon that occurs when a rolling object experiences both rotational and linear motion but also experiences frictional forces that cause slipping. This phenomenon can occur in various situations, such as when a tire rolls on a wet road or a ball rolls on a rough surface.
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Rolling Resistance01:21

Rolling Resistance

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When a solid cylinder rolls steadily on a rigid surface, the normal force applied by the surface on the cylinder is perpendicular to the tangent at the contact point. However, since no materials are entirely rigid, the surface's reaction to the cylinder involves a range of normal pressures.
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Mohr's Circle for Moments of Inertia: Problem Solving01:14

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Mohr's circle is a graphical method for determining an area's principal moments by plotting the moments and product of inertia on a rectangular coordinate system. This circle can also be used to calculate the orientation of the principal axes.
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Rolling Resistance: Problem Solving01:17

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Rolling resistance, also known as rolling friction, is the force that resists the motion of a rolling object, such as a wheel, tire, or ball, when it moves over a surface. It is caused by the deformation of the object and the surface in contact with each other, as well as other factors like internal friction, hysteresis, and energy losses within the materials. Rolling resistance opposes the object's motion, requiring additional energy to overcome it and maintain movement. In practical...
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Related Experiment Video

Updated: Feb 14, 2026

Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples
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Highly sensitive chemiluminescence technology for protein detection using aptamer-based rolling circle amplification

Zhi-Juan Cao1, Qian-Wen Peng1, Xue Qiu1

  • 1School of Pharmacy, Fudan University, Shanghai 201203, China.

Journal of Pharmaceutical Analysis
|February 7, 2018
PubMed
Summary

This study presents a novel chemiluminescent (CL) biosensor for protein detection. Utilizing rolling circle amplification (RCA), this robust platform achieves high sensitivity for protein biomarker analysis.

Keywords:
AptamerChemiluminescenceProteinRolling circle amplification

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Protein assays are crucial for diagnosing clinical diseases.
  • Existing methods often lack sensitivity or require complex instrumentation.
  • There is a need for robust, sensitive, and user-friendly protein detection platforms.

Purpose of the Study:

  • To develop a novel chemiluminescent (CL) platform for highly sensitive protein assays.
  • To utilize rolling circle amplification (RCA) for signal enhancement in a 96-well plate format.
  • To establish a robust and selective biosensor for protein biomarker detection.

Main Methods:

  • Immobilization of antibodies on a 96-well plate.
  • Sandwiching of protein targets with aptamer-primer sequences.
  • Rolling circle amplification (RCA) initiated by the aptamer-primer.
  • Chemiluminescent detection using streptavidin-horseradish peroxidase (SA-HRP).

Main Results:

  • Demonstrated a robust and selective chemiluminescent biosensor.
  • Achieved a highly sensitive detection limit of 10 fM.
  • The platform is easy to establish and utilize, requiring no external light source.

Conclusions:

  • The developed CL platform offers a sensitive and selective method for protein detection.
  • This DNA-based biosensor technology has potential for detecting various clinical disease biomarkers.
  • The approach broadens future perspectives for developing advanced biosensing technologies.