Related Experiment Video
Updated: Feb 14, 2026

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
Published on: January 18, 2020
Fingerprint analysis of placenta polypeptide injection by high performance liquid chromatography
Li Huang1, Xiao-Man Wu2, Yu Ji2
1China Pharmaceutical University, Nanjing 210009, China.
Abstract:
Objective: To develop the representative fingerprint for the quality control of placenta polypeptide injection.
Methods:
The chromatographic separation was performed using a Phenomenex Gemini C18 column (250 mm×4.6 mm, 5 μm) maintained at 30 °C. 0.1% aqueous trifluoroacetic acid (Solvent A) and acetonitrile contained 0.1% TFA (Solvent B) were used as mobile phase with a gradient elution. Detection wavelength was 280 nm with the sample injection volume of 50 μL; the flow rate was 1.0 mL/min. The fingerprints of different samples were investigated by similarity analysis.
Results:
Nine peaks were identified as the characteristic common peaks. The similarities of the fingerprints of the 10 batches of samples were above 0.992.
Conclusion:
This method showed high precision and good repeatability, and provided the basis for the improvement of the quality control of placenta polypeptide injection.
More Related Videos
06:25Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
11:26Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake
Published on: September 21, 2017
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Instrumentation
High-Performance Liquid Chromatography: Elution Process
High-Performance Liquid Chromatography: Types of Detectors
Gas Chromatography: Sample Injection Systems
Two primary injection methods are used...
IR Frequency Region: Fingerprint Region