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Updated: Feb 14, 2026

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
Published on: October 1, 2009
Development and validation of RP-HPLC method for estimation of eplerenone in spiked human plasma
Paraag Gide1, Sandeep Sonawane1, Abhishek Chitnis2
1MET's Institute of Pharmacy, MET League of Colleges, Bhujbal Knowledge City, Nashik 422003, Maharashtra, India.
Abstract:
A rapid and simple high performance liquid chromatography (HPLC) method with a UV detection (241 nm) was developed and validated for estimation of eplerenone from spiked human plasma. The analyte and the internal standard (valdecoxib) were extracted with a mixture of dichloromethane and diethyl ether. The chromatographic separation was performed on a HiQSil C-18HS column (250 mm×4.6 mm, 5 μm) with a mobile phase consisting of acetonitrile:water (50:50, v/v) at flow rate of 1 mL/min. The calibration curve was linear in the range 100-3200 ng/mL and the heteroscedasticity was minimized by using weighted least squares regression with weighting factor 1/X.
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