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Updated: Feb 14, 2026

A Micropatterning Assay for Measuring Cell Chirality
Published on: March 11, 2022
New chiral reverse phase HPLC method for enantioselective analysis of ketorolac using chiral AGP column
Sunil K Dubey1, Jangala Hemanth1, Chiranjeevi Venkatesh K1
1Department of Pharmacy, Birla Institute of Technology & Science, Pilani, India.
Abstract:
A simple, specific, precise, sensitive and rapid reverse phase-HPLC method was developed for determination of ketorolac enantiomers, a potent nonnarcotic analgesic in pharmaceutical formulations. The method was developed on a chiral AGP column. Mobile phase was 0.1 M sodium phosphate buffer (pH 4.5): Isopropanol (98:2, v/v), at a flow rate of 1 mL/min with run time of 15 min. Ultraviolet detection was made at 322 nm. The linearity range was 0.02-10 μg/mL for each of the enantiomers. The mobile phase composition was systematically studied to find the optimum chromatographic conditions. Validation of the method under the conditions selected showed that it was selective and precise and that the detector response was linear function of ketorolac.
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