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Updated: Feb 14, 2026

Author Spotlight: Discovering New Alkaloids in Plants with Advanced Mass Spectrometry Techniques
Published on: March 8, 2024
Simultaneous determination of five diterpenoid alkaloids in Herba Delphinii by HPLC/ELSD
Chao-Zhan Lin1, Si-Min Xie1, Chen-Chen Zhu1
1Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China.
Abstract:
A HPLC-ELSD method was developed and validated for simultaneous determination of five Hetisane-type diterpenoid alkaloids in a Tibetan traditional herbal medicine, "Gebu Dilu" (Herba Delphinii), using a Kromasil C18 column (250 mm×4.6 mm, 5 μm) with the mobile phase consisting of acetonitrile and 0.1% triethylamine in gradient (detected by evaporative light scattering detector). The linear ranges of five compounds were determined and method validation was evaluated completely. The established method is rapid and accurate with high repeatability, and can be applied for the quality control of Herba Delphinii.
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