Related Experiment Video
Updated: Feb 14, 2026

Pyrosequencing for Microbial Identification and Characterization
Published on: August 22, 2013
Identification and characterization of related substances in EVT-401 by hyphenated LC-MS techniques
Binan Zhu1, Yuting Lu1, Leilin Chen2
1Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education; Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China.
Abstract:
A sensitive and selective method was developed for the separation and characterization of related substances (RSs) in EVT-401 by hyphenated LC-MS techniques. Complete separation of the RSs was achieved with an Inertsil ODS-SP column (250 mm×4.6 mm, 5 µm) by linear gradient elution using a mobile phase consisting of 0.2% formic acid solution, methanol and acetonitrile. EVT-401 was found to be susceptible to acid, alkaline and oxidative stresses, while relatively stable under photolytic and thermal dry stress conditions. Fourteen RSs including six process-related substances and eight degradation products were detected and identified in EVT-401 with positive ESI high-resolution TOF-MS analysis of their parent ions and the corresponding product mass spectra elucidation, and some of them were further verified by chemical synthesis and NMR spectroscopy. The specific LC-MS method developed for separation, identification and characterization of RSs is valuable for EVT-401 manufacturing process optimization and quality control.
More Related Videos
Related Concept Videos
Substance Use Disorders Affecting Sleep
Understanding the concepts of physical dependence,...
Deleterious Substances in Aggregate
Another type of impurity is clay and fine material that...
Connective Tissue Fibers and Ground Substance
Methods of Classification and Identification
Pharmacogenomics: Identification of New Drug Targets
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

