Related Experiment Video
Updated: Feb 14, 2026

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
Isolation, characterization and chromatography based purification of antibacterial compound isolated from rare
Ravi Ranjan1, Vasantba Jadeja2
1Department of Biotechnology, Shree M. & N. Virani Science College, Kalawad Road, Rajkot 360005, Gujarat, India.
Abstract:
Endophytic actinomycetes are considered as one of the relatively unexplored potential sources in search of antibiotic producer against antibiotic resistant pathogens. A potent strain isolated from Catharanthus roseus that displays antibacterial potential against antibiotic resistant human pathogen Staphylococcus aureus was characterized and designated as Micrococcus yunnanensis strain rsk5. Rsk5 is capable of producing optimum antibacterial metabolites on starch casein medium at 30 °C, pH 5 and 2% NaCl condition. The crude antibacterial agent was extracted from fermentation broth by ethyl acetate and separated by TLC using chloroform-methanol (24:1, v/v) solvent system with Rf value of 0.26. It was partially purified by flash chromatography, followed by HPLC and analyzed by ultraviolet visible spectrophotometer to get absorption maxima at 208.4 nm. The ESI-MS spectra showed molecular ion peaks at m/z 472.4 [M-H], which does not match with any known antibacterial compound.
Related Concept Videos
DNA Isolation
DNA Isolation
Solubility of Ionic Compounds
Organic Compounds
Molecules and Compounds
Coordination Compounds and Nomenclature

