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Localization of Engineered Vasculature within 3D Tissue Constructs.

Shira Landau1, Shaowei Guo1, Shulamit Levenberg1

  • 1Department of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa, Israel.

Frontiers in Bioengineering and Biotechnology
|February 7, 2018
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Summary

Endothelial cells form blood vessels within scaffolds, developing distinct supporting cell phenotypes and spatially dependent Yes-associated protein (YAP) expression, advancing engineered microvasculature research.

Keywords:
3D scaffoldsangiogenesisblood vesselsmigrationpericytes

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Tissue Engineering

Background:

  • In vitro vessel network systems are crucial for studying angiogenesis and vasculogenesis.
  • Optimizing engineered microvasculature requires understanding cell behavior cues and timelines.

Purpose of the Study:

  • To investigate endothelial cell behavior and vessel formation within a 3D scaffold.
  • To characterize the phenotypes of supporting cells and the expression of Yes-associated protein (YAP) in relation to vessel proximity and scaffold depth.

Main Methods:

  • Utilized in vitro vessel network systems and 3D scaffolds.
  • Observed endothelial cell migration and blood vessel formation.
  • Analyzed supporting cell phenotypes based on location (adjacent to vessels vs. scaffold surface).
  • Assessed spatial expression patterns of Yes-associated protein (YAP).

Main Results:

  • Endothelial cells migrated into the scaffold, forming blood vessels.
  • Supporting cells exhibited localization-dependent phenotypes: smooth muscle-like near vessels and pericyte-like on the surface.
  • Nuclear YAP expression was higher in cells on the scaffold surface compared to those deeper within.

Conclusions:

  • Demonstrated endothelial cell-driven blood vessel formation within a 3D scaffold.
  • Revealed distinct, location-dependent supporting cell differentiation.
  • Highlighted spatially regulated YAP expression, offering insights into controlling cell behavior for improved engineered vascular networks.