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Author Spotlight: Studying Behavior of Acanthamoeba to Develop Targeted Strategies for Preventing Acanthamoeba Keratitis
Published on: September 20, 2024
An improvised medium for axenic cultivation of Acanthamoeba spp
Kirti Megha1, Amit Gupta2, Rakesh Sehgal1
1Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Abstract:
Acanthamoebae can be easily grown in bacterised cultures, but their growth in axenic media is tedious and many times unsuccessful. We thus experimented with some additives in the conventional axenic medium for growth of various isolates of Acanthamoeba. Addition of Torula yeast RNA was found to significantly enhance the growth of Acanthamoebae in the axenic culture medium.
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