Supplemented stallion seminal plasma can improve impaired motility due to the dilution effect in chilled Asian

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A Pinyopummin1, S Mahasawangkul2, G Nunklang1

  • 1Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom, Thailand.

Reproduction in Domestic Animals = Zuchthygiene
|February 7, 2018
PubMed

Insights

Diluting Asian elephant sperm affects motility. Restoring with stallion seminal plasma (SP) improved motility in highly diluted chilled sperm, unlike elephant SP. This finding aids elephant conservation efforts.

Area of Science:

  • Veterinary reproduction
  • Animal conservation science
  • Sperm cryopreservation

Background:

  • Seminal plasma (SP) plays a crucial role in sperm function.
  • Understanding dilution effects is vital for effective sperm preservation in endangered species.
  • Asian elephant (Elephas maximus) sperm require specific handling for artificial insemination.

Purpose of the Study:

  • To investigate the dilution effect on chilled Asian elephant sperm motility and integrity.
  • To determine the efficacy of restoring seminal plasma (SP) proportion in diluted semen.
  • To compare the effects of elephant SP versus stallion SP on sperm quality.

Main Methods:

  • Semen from eight male Asian elephants was collected and evaluated.
  • Sperm samples were diluted with Tris-glucose-egg yolk extender (TE) at various ratios (1:1, 1:3, 1:7, 1:15).
  • Diluted semen was supplemented with either elephant SP or stallion SP to assess restoration effects on sperm motility, viability, and acrosome integrity after 48 hours of cooling.

Main Results:

  • Sperm motility and velocity were significantly higher in 1:1 dilutions compared to 1:7 and 1:15 dilutions after 48 hours.
  • Restoring diluted semen (1:7) with stallion SP improved sperm motility velocity, but not overall motility or viability, compared to elephant SP.
  • Restoring highly diluted semen (1:15) with stallion SP significantly enhanced sperm motility and velocity, whereas elephant SP did not show similar improvements.

Conclusions:

  • Asian elephant sperm motility is susceptible to the dilution effect during chilled storage.
  • Stallion seminal plasma, but not elephant seminal plasma, can improve motility in highly diluted, chilled Asian elephant sperm.
  • These findings offer insights for optimizing sperm preservation protocols for Asian elephants.
Keywords:
Asian elephantcooled storagedilution effectsperm

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