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Blockade of miR-663b inhibits cell proliferation and induces apoptosis in osteosarcoma via regulating TP73 expression

Y Shu, W Ye, Y L Gu

    Bratislavske Lekarske Listy
    |February 7, 2018
    PubMed
    Abstract

    Insights

    MicroRNA-663b (miR-663b) promotes osteosarcoma progression by targeting TP73. Down-regulating miR-663b inhibits tumor cell proliferation and induces apoptosis, offering a potential therapeutic strategy for osteosarcoma.

    Area of Science:

    • Oncology
    • Molecular Biology
    • Gene Regulation

    Background:

    • Osteosarcoma (OS) is a primary bone malignancy with significant mortality.
    • MicroRNAs (miRNAs) play crucial roles in cancer development and progression.
    • The specific role of miR-663b in osteosarcoma remains to be fully elucidated.

    Purpose of the Study:

    • To investigate the role of miR-663b in osteosarcoma progression.
    • To explore the underlying molecular mechanisms of miR-663b action in OS.
    • To identify potential therapeutic targets for osteosarcoma.

    Main Methods:

    • Quantitative real-time PCR (qRT-PCR) to determine miR-663b expression.
    • Bioinformatic prediction (TargetScan) and dual-luciferase reporter assay to identify miR-663b targets.
    • Cell proliferation (CCK8) and apoptosis assays (flow cytometry) in miR-663b-inhibited U2OS cells.
    • Western blot and qRT-PCR to analyze target gene and protein expression.

    Main Results:

    • miR-663b directly targets and negatively regulates TP73 expression in osteosarcoma cells.
    • Down-regulation of miR-663b significantly reduced U2OS cell proliferation and increased apoptosis.
    • miR-663b inhibition altered the expression of apoptosis-related proteins (Bcl-2, Bax) and promoted p53 and p21 expression.

    Conclusions:

    • miR-663b promotes osteosarcoma progression by targeting TP73.
    • Down-regulation of miR-663b represses proliferation and induces apoptosis in osteosarcoma.
    • Targeting miR-663b represents a potential therapeutic strategy for osteosarcoma treatment.
    Keywords:
    TP73miR-663bosteosarcomaproliferation apoptosis.

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