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miR-16-1-3p targets TWIST1 to inhibit cell proliferation and invasion in NSCLC

Q Q Feng, Z Q Dong, Y Zhou

    Bratislavske Lekarske Listy
    |February 7, 2018
    PubMed

    Insights

    MicroRNA-16-1-3p (miR-16-1-3p) is downregulated in non-small cell lung cancer (NSCLC). Upregulating miR-16-1-3p inhibits NSCLC cell proliferation and invasion by targeting TWIST1.

    Area of Science:

    • Molecular Biology
    • Oncology

    Background:

    • Non-small cell lung cancer (NSCLC) is a leading cause of cancer-related mortality.
    • Understanding the molecular mechanisms underlying NSCLC progression is crucial for developing effective therapies.

    Purpose of the Study:

    • To investigate the role of miR-16-1-3p in NSCLC.
    • To explore the regulatory relationship between miR-16-1-3p and TWIST1 in NSCLC cells.

    Main Methods:

    • Quantitative real-time PCR (qRT-PCR) and Western blot analysis.
    • MTT, scratch, and Transwell assays for cell proliferation, migration, and invasion.
    • Luciferase reporter assay to confirm TWIST1 as a direct target of miR-16-1-3p.

    Main Results:

    • miR-16-1-3p expression was significantly downregulated in NSCLC cells.
    • Overexpression of miR-16-1-3p inhibited A549 cell proliferation, migration, and invasion.
    • TWIST1 was identified as a direct target gene of miR-16-1-3p, with miR-16-1-3p suppressing its expression.

    Conclusions:

    • miR-16-1-3p acts as a tumor suppressor in NSCLC by inhibiting cell proliferation and invasion.
    • The miR-16-1-3p/TWIST1 axis represents a potential therapeutic target for NSCLC treatment.
    • Further investigation into miR-16-1-3p's role could offer novel strategies for NSCLC management.
    Keywords:
    NSCLCTWIST1invasion target.miR-16-1-3pproliferation

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