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Related Experiment Videos

Immunological evidence for prothrombin in human platelets.

R J Alexander

    Thrombosis and Haemostasis
    |April 30, 1986
    PubMed
    Summary
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    Researchers sought glycoprotein V (GPV) in plasma but found platelets contain prothrombin instead. This study clarifies the source of thrombin-sensitive proteins on platelet surfaces.

    Area of Science:

    • Biochemistry
    • Hematology
    • Immunology

    Background:

    • Platelet activation involves thrombin-sensitive substrates.
    • Glycoprotein V (GPV) was previously reported as a plasma antigen, potentially acting as a platelet substrate.

    Purpose of the Study:

    • To isolate and characterize the platelet surface substrate for thrombin, identified as glycoprotein V (GPV).
    • To determine if GPV is present in plasma as previously suggested.

    Main Methods:

    • Plasma fractionation using platelet GPV purification protocols.
    • Protein purification, electrophoresis, and antiserum production.
    • Immunochemical assays including double diffusion and adsorption studies.

    Main Results:

    Related Experiment Videos

    • A thrombin-sensitive protein was isolated from plasma with similar electrophoretic mobility to platelet GPV.
    • Antiserum against the purified protein reacted with platelet proteins and inhibited thrombin-induced platelet activation.
    • Immunochemical analysis revealed the purified protein was related to prothrombin, not GPV.
    • Antibodies against prothrombin also adsorbed proteins from platelets.

    Conclusions:

    • Plasma does not contain significant amounts of glycoprotein V (GPV).
    • Platelets appear to contain prothrombin or a closely related protein, which functions as the thrombin-sensitive substrate.