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Angiotensin receptor-binding molecule in leukocytes in association with the systemic and leukocyte inflammatory

Kotaro Haruhara1, Hiromichi Wakui2, Kengo Azushima3

  • 1Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Japan; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan.

Atherosclerosis
|February 7, 2018
PubMed

Insights

Leukocyte Angiotensin II type 1 receptor (AT1R)-associated protein (ATRAP) mRNA is abundant in healthy individuals and correlates with inflammation markers in non-communicable diseases (NCDs). ATRAP may act as an anti-inflammatory factor in NCDs.

Area of Science:

  • Immunology
  • Cardiovascular Science
  • Nephrology

Background:

  • The renin-angiotensin system in leukocytes contributes to non-communicable diseases (NCDs) like hypertension and atherosclerosis.
  • Angiotensin II type 1 receptor (AT1R)-associated protein (ATRAP) inhibits pathological AT1R signaling in animal models.
  • Investigating ATRAP expression and regulation in leukocytes is crucial for understanding NCDs.

Purpose of the Study:

  • To investigate the expression and regulation of ATRAP in human leukocytes.
  • To determine the association between leukocyte ATRAP mRNA levels and clinical variables in NCD patients.
  • To explore the role of ATRAP in leukocyte inflammation.

Main Methods:

  • Droplet digital polymerase chain reaction (ddPCR) was used to measure human leukocyte ATRAP mRNA.
  • Leukocyte ATRAP mRNA levels were analyzed in relation to clinical variables in 86 NCD outpatients.
  • Leukocyte cytokine mRNA was examined in bone-marrow ATRAP-deficient and wild-type chimeric mice after lipopolysaccharide injection.

Main Results:

  • ATRAP mRNA was highly expressed in leukocytes, particularly granulocytes and monocytes, of healthy subjects.
  • Leukocyte ATRAP mRNA levels positively correlated with granulocyte, monocyte counts, and C-reactive protein in NCD patients.
  • ATRAP mRNA levels were associated with inflammatory cytokines (IL-1β, TNF-α, MCP-1) in NCD patients' leukocytes. Leukocyte IL-1β was upregulated in ATRAP-deficient mice.

Conclusions:

  • Leukocyte ATRAP serves as a marker for systemic and leukocyte inflammation.
  • ATRAP appears to function as an anti-inflammatory factor in the pathophysiology of NCDs.
  • Further research into ATRAP's role could offer new therapeutic strategies for NCDs.
Abstract

Keywords:
InflammationLeukocyteNon-communicable diseasesReceptorRenin-angiotensin system

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