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Microfluidic paper-based chip platform for benzoic acid detection in food.
Chan-Chiung Liu1, Yao-Nan Wang2, Lung-Ming Fu3
1Department of Food Science, National Pingtung University of Science and Technology, Pingtung 912, Taiwan.
Food Chemistry
|February 7, 2018
Summary
A novel microfluidic paper-based analytical device (µPAD) integrated with a portable system enables accurate benzoic acid detection using the Janovsky reaction. This compact platform offers a reliable alternative to traditional methods for food sample analysis.
Area of Science:
- Analytical Chemistry
- Microfluidics
- Chemical Sensing
Background:
- Benzoic acid is a common food preservative, necessitating reliable detection methods.
- Existing analytical techniques can be complex and time-consuming.
- Microfluidic paper-based analytical devices (µPADs) offer potential for portable and low-cost analysis.
Purpose of the Study:
- To develop an integrated microfluidic platform for benzoic acid concentration detection.
- To utilize the Janovsky reaction for colorimetric analysis of benzoic acid.
- To create a portable and user-friendly system for food sample analysis.
Main Methods:
- An integrated system combining a µPAD with a portable detection unit was designed.
- Benzoic acid was derivatized to 3,5-Dinitrobenzoic acid and reacted on the µPAD.
- Color changes were quantified using a CMOS camera and RGB analysis software on a smartphone.
Main Results:
- The system demonstrated a strong correlation (R²=0.9953) between R(ed)+B(lue) intensity and benzoic acid concentration (500-4000 ppm).
- Analysis of commercial food samples showed a maximum deviation of 6.6% compared to standard HPLC methods.
- The developed platform proved effective for quantifying benzoic acid in real-world samples.
Conclusions:
- The integrated microfluidic paper-based chip platform is a compact and reliable tool for benzoic acid measurement.
- This approach offers a promising alternative for on-site food safety monitoring.
- The system's accuracy and portability enhance its applicability in various settings.
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