Jove
Visualize
Contact Us

Related Experiment Videos

Chromate reduction by rabbit liver aldehyde oxidase.

R B Banks, R T Cooke

    Biochemical and Biophysical Research Communications
    |May 29, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Pernicious anemia and carcinoma of the oesophagus.

    Lancet (London, England)·2010
    Same author

    Fulminating Sonne Dysentery. Report of Two Cases.

    British medical journal·2010
    Same author

    Megalocytic Anaemia in a Young Vegetarian.

    British medical journal·2010
    Same author

    Simmonds's Disease due to Post-partum Necrosis of Anterior Pituitary.

    British medical journal·2010
    Same author

    Amyloid macroglossia.

    Lancet (London, England)·2010
    Same author

    Non-Hodgkin's lymphoma and occupational exposure to hair dyes among people with AIDS.

    American journal of public health·1993
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies

    Rabbit liver aldehyde oxidase reduces chromate when supplied with electron donors like 1-methylnicotinamide. This enzymatic chromate reduction requires both the enzyme and a substrate, indicating aldehyde oxidase

    Area of Science:

    • Biochemistry
    • Enzymology
    • Toxicology

    Background:

    • Chromate is a toxic environmental pollutant.
    • Enzymatic pathways for chromate reduction are not fully understood.
    • Aldehyde oxidase is a key enzyme in xenobiotic metabolism.

    Purpose of the Study:

    • To investigate the role of rabbit liver aldehyde oxidase in chromate reduction.
    • To identify potential electron donors supporting this enzymatic reaction.

    Main Methods:

    • Partially purified rabbit liver aldehyde oxidase was used.
    • Chromate reduction was monitored in the presence of 1-methylnicotinamide and other potential substrates.
    • Enzyme inhibitors specific to aldehyde oxidase and xanthine oxidase were employed.

    Related Experiment Videos

    Main Results:

    • Aldehyde oxidase catalyzed the reduction of chromate.
    • 1-methylnicotinamide and other aldehyde oxidase substrates supported chromate reduction.
    • The reaction required both the enzyme and an electron donor substrate.
    • Inhibitors of aldehyde oxidase, but not xanthine oxidase, affected the reduction rate.

    Conclusions:

    • Rabbit liver aldehyde oxidase is involved in the enzymatic reduction of chromate.
    • The findings suggest a novel detoxification pathway for chromate in the liver.